Thrombomodulin 抗体 (AA 17-517)
Quick Overview for Thrombomodulin 抗体 (AA 17-517) (ABIN326112)
抗原
See all Thrombomodulin (THBD) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 17-517
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特异性
- Recognizes mouse Thrombomodulin.
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纯化方法
- Protein G purified
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免疫原
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NS0-derived rmThrombomodulin. (aa 17 - 517).
Type of Immunogen: Recombinant protein -
亚型
- IgG2b
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应用备注
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Approved: ELISA, Flo (25 μg/mL), ICC (10 μg/mL), IHC, IHC-Fr, WB (1 - 2 μg/mL)
Usage: Suitable for use in Flow Cytometry, ELISA, Western Blot Immunohistochemistry and Immunocytochemistry. Flow Cytometry: 25 μg/mL, Use 10 μL to label 1-2.5x10^5 bEnd.3 cells in a total reaction volume < 200 μL. ELISA (Direct): 0.5-1.0 μg/mL The detection limit is ~5 ng/well. Western Blot: 1-2 μg/mL Using a colorimetric detection system, the detection limit is ~25 ng/lane under non-reducing conditions. Use under reducing conditions is not recommended. Chemiluminescent detection will increase sensitivity by 5-50 fold. Immunohistochemistry: 25 μg/mL. Used with frozen sections of normal mouse kidney and heart fixed with 85 mM Na2HPO4, 75 mM KH2P04, 4 % paraformaldehyde, and 14 % (v/v) saturated picric acid, pH 6.9. Immunocytochemistry: 10 μg/mL. Used with bEnd.3 cells. Cells were fixed with PBS, 4 % paraformaldehyde and blocked with PBS, 10 % normal donkey serum, 0.1 % Triton X-100, 1 % BSA. Between each step, cells were washed with PBS, BSA. -
说明
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Target Species of Antibody: Mouse
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- 200 μL sterile PBS
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浓度
- Lot specific
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缓冲液
- Lyophilized from PBS, 5 % trehalose.
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注意事项
- Aviod frequent freeze thaws.
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储存条件
- -20 °C
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储存方法
- Store at -20°C prior to reconstitution. After reconstitution aliquot and freeze at-20°C. Avoid freeze-thaw cycles.
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- Thrombomodulin (THBD)
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别名
- THBD / CD141 / Thrombomodulin
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背景
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Name/Gene ID: THBD
Synonyms: THBD, BDCA3, CD141 antigen, CD141, AHUS6, Fetomodulin, THPH12, TM, THRM, Thrombomodulin -
基因ID
- 7056
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UniProt
- P07204
抗原
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