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Thrombomodulin 抗体 (AA 17-517)

This anti-Thrombomodulin antibody is a 大鼠 单克隆 antibody detecting Thrombomodulin in WB, IHC, ELISA, FACS, ICC 和 IHC (fro). Suitable for 小鼠.
产品编号 ABIN326112
发货至: 中国

Quick Overview for Thrombomodulin 抗体 (AA 17-517) (ABIN326112)

抗原

See all Thrombomodulin (THBD) 抗体
Thrombomodulin (THBD)

适用

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小鼠

宿主

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大鼠

克隆类型

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单克隆

标记

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This Thrombomodulin antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

克隆位点

461714
  • 抗原表位

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    AA 17-517

    特异性

    Recognizes mouse Thrombomodulin.

    纯化方法

    Protein G purified

    免疫原

    NS0-derived rmThrombomodulin. (aa 17 - 517).

    Type of Immunogen: Recombinant protein

    亚型

    IgG2b
  • 应用备注

    Approved: ELISA, Flo (25 μg/mL), ICC (10 μg/mL), IHC, IHC-Fr, WB (1 - 2 μg/mL)

    Usage: Suitable for use in Flow Cytometry, ELISA, Western Blot Immunohistochemistry and Immunocytochemistry. Flow Cytometry: 25 μg/mL, Use 10 μL to label 1-2.5x10^5 bEnd.3 cells in a total reaction volume < 200 μL. ELISA (Direct): 0.5-1.0 μg/mL The detection limit is ~5 ng/well. Western Blot: 1-2 μg/mL Using a colorimetric detection system, the detection limit is ~25 ng/lane under non-reducing conditions. Use under reducing conditions is not recommended. Chemiluminescent detection will increase sensitivity by 5-50 fold. Immunohistochemistry: 25 μg/mL. Used with frozen sections of normal mouse kidney and heart fixed with 85 mM Na2HPO4, 75 mM KH2P04, 4 % paraformaldehyde, and 14 % (v/v) saturated picric acid,  pH 6.9. Immunocytochemistry: 10 μg/mL. Used with bEnd.3 cells. Cells were fixed with PBS, 4 % paraformaldehyde and blocked with PBS, 10 % normal donkey serum, 0.1 % Triton X-100, 1 % BSA. Between each step, cells were washed with PBS, BSA.

    说明

    Target Species of Antibody: Mouse

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    200 μL sterile PBS

    浓度

    Lot specific

    缓冲液

    Lyophilized from PBS, 5 % trehalose.

    注意事项

    Aviod frequent freeze thaws.

    储存条件

    -20 °C

    储存方法

    Store at -20°C prior to reconstitution. After reconstitution aliquot and freeze at-20°C. Avoid freeze-thaw cycles.
  • 抗原

    Thrombomodulin (THBD)

    别名

    THBD / CD141 / Thrombomodulin

    背景

    Name/Gene ID: THBD

    Synonyms: THBD, BDCA3, CD141 antigen, CD141, AHUS6, Fetomodulin, THPH12, TM, THRM, Thrombomodulin

    基因ID

    7056

    UniProt

    P07204
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