PDCD1LG2 抗体
Quick Overview for PDCD1LG2 抗体 (ABIN325068)
抗原
See all PDCD1LG2 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Recognizes mouse PD-L2. In direct ELISAs and Western Blots, this antibody shows ~20 % cross-reactivity with rhPD-L2.
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纯化方法
- Immunoaffinity purified
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免疫原
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NS0-derived rmPD-L2.
Type of Immunogen: Recombinant protein -
亚型
- IgG
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anti-Programmed Cell Death 1 Ligand 2 (PDCD1LG2) (AA 21-118) antibody
PDCD1LG2 适用: 人 WB, FACS, IHC, ELISA, IP, IF 宿主: 小鼠 Monoclonal 7F11D11 unconjugated
anti-Programmed Cell Death 1 Ligand 2 (PDCD1LG2) (AA 27-220) antibodyPDCD1LG2 适用: 人 WB, FACS, ELISA, IF, Coat 宿主: 小鼠 Monoclonal PDL2-2676 unconjugated
anti-Programmed Cell Death 1 Ligand 2 (PDCD1LG2) (AA 33-236) antibodyPDCD1LG2 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Programmed Cell Death 1 Ligand 2 (PDCD1LG2) (AA 36-219) antibodyPDCD1LG2 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Programmed Cell Death 1 Ligand 2 (PDCD1LG2) antibodyPDCD1LG2 适用: 人 FACS, IHC 宿主: 小鼠 Monoclonal 24F-10C12 unconjugated
anti-Programmed Cell Death 1 Ligand 2 (PDCD1LG2) antibody (PE)PDCD1LG2 适用: 人 FACS 宿主: 小鼠 Monoclonal 24F-10C12 PE
anti-Programmed Cell Death 1 Ligand 2 (PDCD1LG2) (AA 21-268) antibodyPDCD1LG2 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Programmed Cell Death 1 Ligand 2 (PDCD1LG2) (AA 22-111) antibodyPDCD1LG2 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 7D5 unconjugated
anti-Programmed Cell Death 1 Ligand 2 (PDCD1LG2) (AA 33-236) antibodyPDCD1LG2 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C7 unconjugated
anti-Programmed Cell Death 1 Ligand 2 (PDCD1LG2) (AA 40-75), (N-Term) antibodyPDCD1LG2 适用: 人 WB, IHC (p) 宿主: 小鼠 Monoclonal 2011CT786-31-38 unconjugated
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应用备注
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Approved: ELISA, Flo, Func, IHC, WB
Usage: Western Blot, Immunohistochemistry, Flow Cytometry, Blocking of receptor-ligand interaction, Direct ELISA. Western Blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect mouse PD-L2. The detection limit for rmPD-L2 is approximately 2 ng/lane under non-reducing and reducing conditions. Immunohistochemistry: This antibody will detect PD-L2 in cells and tissues. The working dilution is 2-15 μg/mL. Flow Cytometry: This antibody has been tested on Tim-1 stimulated RAW 264.7 cells for use in flow cytometry. Dilute this antibody to 50 μg/mL and add 10 μL of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200 μL. The binding of unlabeled antibodies may be visualized by adding 10 μL of a 25 μg/mL stock solution of a secondary developing reagent such as anti-goat IgG conjugated to a fluorochrome. Blocking of receptor-ligand interaction: Approximately 1-4 μg/mL of this antibody will block 50 % of the binding of 1 μg/mL of rmPD-1 to immobilized rmPD-L2/Fc chimera (100 μL of a 1 μg/mL solution was coated in each well) in a functional ELISA. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect mouse PD-L2. The detection limit for rmPD-L2 is approximately 1.2 ng/well. -
说明
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Target Species of Antibody: Mouse
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitution: Reconstitute, sterile PBS. If 1 mL of PBS is used, the antibody concentration will be 100 μg/mL.
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浓度
- Lot specific
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缓冲液
- Lyophilized from PBS, 5 % trehalose. Reconstitution: Reconstitute, sterile PBS. If 1 mL of PBS is used, the antibody concentration will be 100 μg/mL.
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- PDCD1LG2 (Programmed Cell Death 1 Ligand 2 (PDCD1LG2))
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别名
- PDCD1LG2 / CD273 / PD-L2
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背景
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Name/Gene ID: PDCD1LG2
Synonyms: PDCD1LG2, B7 dendritic cell molecule, B7-DC, B7DC, BA574F11.2, CD273, Btdc, CD273 antigen, PD-L2, PDCD1 ligand 2, PD-1-ligand 2, Programmed death ligand 2, Butyrophilin B7-DC, PD-1 ligand 2, PDCD1L2, PDL2 -
基因ID
- 80380
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UniProt
- Q9BQ51
抗原
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