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NUMB 抗体

The 绵羊 多克隆 anti-NUMB antibody is suitable to detect NUMB in samples from 人. It has been validated for WB, ELISA 和 ICC.
产品编号 ABIN324854
发货至: 中国
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中国
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
4A Biotech Co.,Ltd.
Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for NUMB 抗体 (ABIN324854)

抗原

See all NUMB 抗体
NUMB (Numb Homolog (NUMB))

适用

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宿主

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绵羊

克隆类型

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多克隆

标记

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This NUMB antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Immunocytochemistry (ICC)
  • 特异性

    Recognizes human Numb. Species sequence homology: mouse: 88 % and canine: 90 %.

    纯化方法

    Immunoaffinity purified

    免疫原

    E. coli-derived recombinant human rhNumb.

    Type of Immunogen: Recombinant protein

    亚型

    IgG
  • 应用备注

    Approved: ELISA (0.5 - 1 μg/mL), ICC (10 μg/mL), WB (1 μg/mL)

    Usage: Suitable for use in Western blot, Immunocytochemistry, and Direct ELISA. Western blot: 1.0 μg/mL. Immunocytochemistry: 10 μg/mL Fix cells with PBS containing 4 % paraformaldehyde for 20 minutes at RT and block with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA for 45 minutes at RT. After blocking, incubate cells with diluted primary antibody for 3 hours at RT. Follow by NorthernLights 557 donkey anti-sheep lgG at RT for 1 hour. Between each step, wash cells with PBS containing 0.1 % BSA. Direct ELISA: 0.5-1.0 μg/mL. The detection limit is ~0.1 ng/well.

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Reconstitute, sterile PBS to a concentration of 0.5 mg/mL.

    浓度

    Lot specific

    缓冲液

    Lyophilized from PBS, 5 % trehalose. Reconstitute, sterile PBS to a concentration of 0.5 mg/mL.
  • 抗原

    NUMB (Numb Homolog (NUMB))

    别名

    NUMB

    背景

    Name/Gene ID: NUMB

    Synonyms: NUMB, C14_5527, Numb (Drosophila) homolog, S171, Protein numb homolog, C14orf41, H-Numb, Numb homolog (Drosophila), Protein S171

    基因ID

    8650

    UniProt

    P49757

    途径

    Cell-Cell Junction Organization
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