NUMB 抗体
Quick Overview for NUMB 抗体 (ABIN324854)
抗原
See all NUMB 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Recognizes human Numb. Species sequence homology: mouse: 88 % and canine: 90 %.
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纯化方法
- Immunoaffinity purified
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免疫原
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E. coli-derived recombinant human rhNumb.
Type of Immunogen: Recombinant protein -
亚型
- IgG
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anti-Numb Homolog (NUMB) (C-Term) antibody
Verified NUMB 适用: 人, 小鼠 IHC, ELISA, IF, FACS 宿主: 山羊 Polyclonal unconjugated
anti-Numb Homolog (NUMB) (AA 521-640) antibodyNUMB 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Numb Homolog (NUMB) (AA 1-50) antibodyNUMB 适用: 人, 小鼠, 大鼠, 猴, Cow WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Numb Homolog (NUMB) (AA 1-135) antibodyNUMB 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 4A7-A6 unconjugated
anti-Numb Homolog (NUMB) (pSer276) antibodyNUMB 适用: 小鼠 WB, ELISA, IF (cc), IF (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Numb Homolog (NUMB) (C-Term) antibodyNUMB 适用: 人, 小鼠, 大鼠, 小鸡 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Numb Homolog (NUMB) (AA 1-214) antibodyNUMB 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Numb Homolog (NUMB) (AA 1-135) antibodyNUMB 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated
anti-Numb Homolog (NUMB) (AA 376-482) antibodyNUMB 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Numb Homolog (NUMB) (AA 377-642) antibodyNUMB 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Approved: ELISA (0.5 - 1 μg/mL), ICC (10 μg/mL), WB (1 μg/mL)
Usage: Suitable for use in Western blot, Immunocytochemistry, and Direct ELISA. Western blot: 1.0 μg/mL. Immunocytochemistry: 10 μg/mL Fix cells with PBS containing 4 % paraformaldehyde for 20 minutes at RT and block with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA for 45 minutes at RT. After blocking, incubate cells with diluted primary antibody for 3 hours at RT. Follow by NorthernLights 557 donkey anti-sheep lgG at RT for 1 hour. Between each step, wash cells with PBS containing 0.1 % BSA. Direct ELISA: 0.5-1.0 μg/mL. The detection limit is ~0.1 ng/well. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitute, sterile PBS to a concentration of 0.5 mg/mL.
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浓度
- Lot specific
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缓冲液
- Lyophilized from PBS, 5 % trehalose. Reconstitute, sterile PBS to a concentration of 0.5 mg/mL.
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- NUMB (Numb Homolog (NUMB))
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别名
- NUMB
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背景
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Name/Gene ID: NUMB
Synonyms: NUMB, C14_5527, Numb (Drosophila) homolog, S171, Protein numb homolog, C14orf41, H-Numb, Numb homolog (Drosophila), Protein S171 -
基因ID
- 8650
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UniProt
- P49757
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途径
- Cell-Cell Junction Organization
抗原
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