2B4 抗体 (Extracellular Domain)
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- 抗原 See all 2B4 (CD244) 抗体
- 2B4 (CD244) (Natural Killer Cell Receptor 2B4 (CD244))
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抗原表位
- Extracellular Domain
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适用
- 小鼠
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This 2B4 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF)
- 特异性
- Recognizes mouse 2B4 extracellular domain.
- 纯化方法
- Immunoaffinity purified
- 免疫原
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NS0-derived rm2B4 extracellular domain.
Type of Immunogen: Recombinant protein - 亚型
- IgG
- Top Product
- Discover our top product CD244 Primary Antibody
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- 应用备注
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Approved: ELISA, Flo, IF, IHC, WB
Usage: Direct ELISA, Western Blot, Flow Cytometry, Immunohistochemistry. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect mouse 2B4. The detection limit for rm2B4 is approximately 0.3 ng/well. In this format, this antibody shows approximately 5 % cross-reactivity with rh2B4 and less than 1 % cross-reactivity with rhSLAM. Western Blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect mouse 2B4. The detection limit for rm2B4 is approximately 5 ng/lane under non-reducing and reducing conditions. Flow Cytometry: This antibody can be used at 3-10 μg/mL/1 x106 with an appropriate secondary antibody for indirect immunofluorescence staining of cells by flow cytometry. Immunohistochemistry: This antibody will detect 2B4 in cells and tissues. The working dilution is 5-15 μg/mL. - 说明
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Target Species of Antibody: Mouse
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Reconstitution: Reconstitute, sterile PBS. If 1 mL of PBS is used, the antibody concentration will be 500 μg/mL.
- 浓度
- Lot specific
- 缓冲液
- Lyophilized from PBS, 5 % trehalose. Reconstitution: Reconstitute, sterile PBS. If 1 mL of PBS is used, the antibody concentration will be 500 μg/mL.
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