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CD48 抗体

CD48 适用: 人 FACS, WB, ELISA 宿主: 小鼠 Monoclonal unconjugated
产品编号 ABIN323355
发货至: 中国
  • 抗原 See all CD48 抗体
    CD48
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    宿主
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    小鼠
    克隆类型
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    单克隆
    标记
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    This CD48 antibody is un-conjugated
    应用范围
    • 82
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    Flow Cytometry (FACS), Western Blotting (WB), ELISA
    特异性
    Recognizes human CD48. In direct ELISAs and Western Blots this antibody shows no cross-reactivity with rmCD48 or rhOX40.
    纯化方法
    Protein G purified
    免疫原
    NS0-derived rhCD48.

    Type of Immunogen: Recombinant protein
    亚型
    IgG2b
    Top Product
    Discover our top product CD48 Primary Antibody
  • 应用备注
    Approved: ELISA, Flo, WB

    Usage: Western Blot, Flow Cytometry, Direct ELISA. Western Blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect human CD48. Using a colorimetric detection system, the detection limit for rhCD48 is approximately 5 ng/lane under non-reducing conditions. Use of this antibody under reducing conditions is not recommended. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. Flow Cytometry: This antibody was validated for flow cytometry using blood-derived monocytes. Dilute this antibody to 25 μg/mL and add 10 μL of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200 μL. The binding of unlabeled monoclonal antibodies may be visualized by adding a secondary developing reagent such as goat anti-mouse IgG conjugated to a fluorochrome. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect human CD48. The detection limit for rhCD48 is approximately 10 ng/well.
    说明

    Target Species of Antibody: Human

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    Reconstitution: Reconstitute, sterile PBS. If 0.2 mL of PBS is used, the antibody concentration will be 500 μg/mL.
    浓度
    Lot specific
    缓冲液
    Lyophilized from PBS, 5 % trehalose. Reconstitution: Reconstitute, sterile PBS. If 0.2 mL of PBS is used, the antibody concentration will be 500 μg/mL.
  • 抗原
    CD48
    别名
    CD48 (CD48 产品)
    背景
    Name/Gene ID: CD48

    Synonyms: CD48, BCM1, BCM1 surface antigen, BLAST, BLAST1, CD48 Molecule, MEM-102, MCD48, SLAMF2, CD48 antigen, HCD48, Leukocyte antigen MEM-102, TCT.1
    基因ID
    962
    UniProt
    P09326
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