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CD48 抗体

The 小鼠 单克隆 anti-CD48 antibody (ABIN323355) specifically detects CD48 in FACS, WB 和 ELISA. The antibody is reactive with 人 samples.
产品编号 ABIN323355
发货至: 中国
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中国
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
4A Biotech Co.,Ltd.
Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for CD48 抗体 (ABIN323355)

抗原

See all CD48 抗体
CD48

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This CD48 antibody is un-conjugated

应用范围

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Flow Cytometry (FACS), Western Blotting (WB), ELISA
  • 特异性

    Recognizes human CD48. In direct ELISAs and Western Blots this antibody shows no cross-reactivity with rmCD48 or rhOX40.

    纯化方法

    Protein G purified

    免疫原

    NS0-derived rhCD48.

    Type of Immunogen: Recombinant protein

    亚型

    IgG2b
  • 应用备注

    Approved: ELISA, Flo, WB

    Usage: Western Blot, Flow Cytometry, Direct ELISA. Western Blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect human CD48. Using a colorimetric detection system, the detection limit for rhCD48 is approximately 5 ng/lane under non-reducing conditions. Use of this antibody under reducing conditions is not recommended. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. Flow Cytometry: This antibody was validated for flow cytometry using blood-derived monocytes. Dilute this antibody to 25 μg/mL and add 10 μL of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200 μL. The binding of unlabeled monoclonal antibodies may be visualized by adding a secondary developing reagent such as goat anti-mouse IgG conjugated to a fluorochrome. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect human CD48. The detection limit for rhCD48 is approximately 10 ng/well.

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Reconstitution: Reconstitute, sterile PBS. If 0.2 mL of PBS is used, the antibody concentration will be 500 μg/mL.

    浓度

    Lot specific

    缓冲液

    Lyophilized from PBS, 5 % trehalose. Reconstitution: Reconstitute, sterile PBS. If 0.2 mL of PBS is used, the antibody concentration will be 500 μg/mL.
  • 抗原

    CD48

    别名

    CD48

    背景

    Name/Gene ID: CD48

    Synonyms: CD48, BCM1, BCM1 surface antigen, BLAST, BLAST1, CD48 Molecule, MEM-102, MCD48, SLAMF2, CD48 antigen, HCD48, Leukocyte antigen MEM-102, TCT.1

    基因ID

    962

    UniProt

    P09326
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