CD48 抗体
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- 抗原 See all CD48 抗体
- CD48
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CD48 antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Western Blotting (WB), ELISA
- 特异性
- Recognizes human CD48. In direct ELISAs and Western Blots this antibody shows no cross-reactivity with rmCD48 or rhOX40.
- 纯化方法
- Protein G purified
- 免疫原
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NS0-derived rhCD48.
Type of Immunogen: Recombinant protein - 亚型
- IgG2b
- Top Product
- Discover our top product CD48 Primary Antibody
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- 应用备注
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Approved: ELISA, Flo, WB
Usage: Western Blot, Flow Cytometry, Direct ELISA. Western Blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect human CD48. Using a colorimetric detection system, the detection limit for rhCD48 is approximately 5 ng/lane under non-reducing conditions. Use of this antibody under reducing conditions is not recommended. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. Flow Cytometry: This antibody was validated for flow cytometry using blood-derived monocytes. Dilute this antibody to 25 μg/mL and add 10 μL of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200 μL. The binding of unlabeled monoclonal antibodies may be visualized by adding a secondary developing reagent such as goat anti-mouse IgG conjugated to a fluorochrome. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect human CD48. The detection limit for rhCD48 is approximately 10 ng/well. - 说明
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Target Species of Antibody: Human
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Reconstitution: Reconstitute, sterile PBS. If 0.2 mL of PBS is used, the antibody concentration will be 500 μg/mL.
- 浓度
- Lot specific
- 缓冲液
- Lyophilized from PBS, 5 % trehalose. Reconstitution: Reconstitute, sterile PBS. If 0.2 mL of PBS is used, the antibody concentration will be 500 μg/mL.
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