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CCR7 抗体

CCR7 适用: 小鼠 FACS, IHC, Func, Neut 宿主: 大鼠 Monoclonal unconjugated
产品编号 ABIN322907
发货至: 中国
  • 抗原 See all CCR7 抗体
    CCR7 (Chemokine (C-C Motif) Receptor 7 (CCR7))
    适用
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    小鼠
    宿主
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    大鼠
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    单克隆
    标记
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    This CCR7 antibody is un-conjugated
    应用范围
    • 50
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    Flow Cytometry (FACS), Immunohistochemistry (IHC), Functional Studies (Func), Neutralization (Neut)
    特异性
    Recognizes mouse CCR7.
    纯化方法
    Protein G purified
    免疫原
    RBC-2H3 cells expressing mCCR7.

    Type of Immunogen: Cells
    亚型
    IgG2a
    Top Product
    Discover our top product CCR7 Primary Antibody
  • 应用备注
    Approved: Flo, Func, IHC (25 μg/mL), Neut

    Usage: Flow cytometry, Immunohistochemistry, Neutralization of Bioactivity. Flow Cytometry-Dilute this antibody to 25 μg/mL and add 10 μL of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200 μL. The binding of unlabeled monoclonal antibodies may be visualized by adding 10 μL of a 25 μg/mL stock solution of a secondary developing reagent such as goat anti-rat IgG conjugated to a fluorochrome. Immunohistochemistry: 25 μg/mL with appropriate secondary reagents to detect CCR7 on paraformaldehyde-fixed mouse splenocytes. Neutralization of Murine CCR7 Bioactivity: The exact concentration of antibody required to neutralize mouse CCR7 activity is dependent on the cytokine concentration, cell type, growth conditions and the type of activity studied. The Neutralization Dose50 (ND50) for this antibody is defined as that concentration of antibody required to yield one-half maximal inhibition of the cytokine activity on a responsive cell line, when that cytokine is present at a concentration just high enough to elicit a maximum response. The ND50 for this lot of anti-mouse CCR7 antibody was determined to be approximately 2-10 μg/mL in the presence of 25 ng/mL of r  mMIP-3, using the BaF/3 mCCR7 cell line in a chemoattraction assay.
    说明

    Target Species of Antibody: Mouse

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    Sterile PBS
    浓度
    Lot specific
    缓冲液
    Lyophilized from PBS,  pH 7.4, 5 % trehalose
    注意事项
    Avoid freeze-thaw cycles.
    储存条件
    4 °C,-20 °C
    储存方法
    Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
  • 抗原
    CCR7 (Chemokine (C-C Motif) Receptor 7 (CCR7))
    别名
    CCR7 (CCR7 产品)
    别名
    zgc:165629 antibody, ccr7-a antibody, CD197 antibody, Cdw197 antibody, Cmkbr7 antibody, EBI1 antibody, Ebi1h antibody, BLR2 antibody, CDw197 antibody, CMKBR7 antibody, chemokine (C-C motif) receptor 7 antibody, C-C motif chemokine receptor 7 antibody, C-C motif chemokine receptor 7 L homeolog antibody, ccr7 antibody, CCR7 antibody, ccr7.L antibody, Ccr7 antibody
    背景
    Name/Gene ID: CCR7
    Subfamily: Chemokine
    Family: GPCR

    Synonyms: CCR7, BLR2, Chemokine (C-C) receptor 7, CD197, C-C CKR-7, CMKBR7, CC chemokine receptor 7, CCR-7, CD197 antigen, CDw197, EBI1, EVI1, MIP-3 beta receptor, C-c chemokine receptor 7, C-C chemokine receptor type 7, CC-CKR-7, Chemokine c-c motif receptor 7
    基因ID
    1236
    UniProt
    P32248
    途径
    Regulation of Actin Filament Polymerization, Positive Regulation of Immune Effector Process
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