CCR7 抗体
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- 抗原 See all CCR7 抗体
- CCR7 (Chemokine (C-C Motif) Receptor 7 (CCR7))
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适用
- 小鼠
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宿主
- 大鼠
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克隆类型
- 单克隆
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标记
- This CCR7 antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Immunohistochemistry (IHC), Functional Studies (Func), Neutralization (Neut)
- 特异性
- Recognizes mouse CCR7.
- 纯化方法
- Protein G purified
- 免疫原
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RBC-2H3 cells expressing mCCR7.
Type of Immunogen: Cells - 亚型
- IgG2a
- Top Product
- Discover our top product CCR7 Primary Antibody
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- 应用备注
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Approved: Flo, Func, IHC (25 μg/mL), Neut
Usage: Flow cytometry, Immunohistochemistry, Neutralization of Bioactivity. Flow Cytometry-Dilute this antibody to 25 μg/mL and add 10 μL of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200 μL. The binding of unlabeled monoclonal antibodies may be visualized by adding 10 μL of a 25 μg/mL stock solution of a secondary developing reagent such as goat anti-rat IgG conjugated to a fluorochrome. Immunohistochemistry: 25 μg/mL with appropriate secondary reagents to detect CCR7 on paraformaldehyde-fixed mouse splenocytes. Neutralization of Murine CCR7 Bioactivity: The exact concentration of antibody required to neutralize mouse CCR7 activity is dependent on the cytokine concentration, cell type, growth conditions and the type of activity studied. The Neutralization Dose50 (ND50) for this antibody is defined as that concentration of antibody required to yield one-half maximal inhibition of the cytokine activity on a responsive cell line, when that cytokine is present at a concentration just high enough to elicit a maximum response. The ND50 for this lot of anti-mouse CCR7 antibody was determined to be approximately 2-10 μg/mL in the presence of 25 ng/mL of r mMIP-3, using the BaF/3 mCCR7 cell line in a chemoattraction assay. - 说明
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Target Species of Antibody: Mouse
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Sterile PBS
- 浓度
- Lot specific
- 缓冲液
- Lyophilized from PBS, pH 7.4, 5 % trehalose
- 注意事项
- Avoid freeze-thaw cycles.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- 抗原
- CCR7 (Chemokine (C-C Motif) Receptor 7 (CCR7))
- 别名
- CCR7 (CCR7 产品)
- 背景
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Name/Gene ID: CCR7
Subfamily: Chemokine
Family: GPCR
Synonyms: CCR7, BLR2, Chemokine (C-C) receptor 7, CD197, C-C CKR-7, CMKBR7, CC chemokine receptor 7, CCR-7, CD197 antigen, CDw197, EBI1, EVI1, MIP-3 beta receptor, C-c chemokine receptor 7, C-C chemokine receptor type 7, CC-CKR-7, Chemokine c-c motif receptor 7 - 基因ID
- 1236
- UniProt
- P32248
- 途径
- Regulation of Actin Filament Polymerization, Positive Regulation of Immune Effector Process
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