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CCR7 抗体

This anti-CCR7 antibody is a 大鼠 单克隆 antibody detecting CCR7 in FACS, IHC, Func 和 Neut. Suitable for 小鼠.
产品编号 ABIN322907
发货至: 中国

Quick Overview for CCR7 抗体 (ABIN322907)

抗原

See all CCR7 抗体
CCR7 (Chemokine (C-C Motif) Receptor 7 (CCR7))

适用

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小鼠

宿主

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大鼠

克隆类型

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单克隆

标记

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This CCR7 antibody is un-conjugated

应用范围

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Flow Cytometry (FACS), Immunohistochemistry (IHC), Functional Studies (Func), Neutralization (Neut)
  • 特异性

    Recognizes mouse CCR7.

    纯化方法

    Protein G purified

    免疫原

    RBC-2H3 cells expressing mCCR7.

    Type of Immunogen: Cells

    亚型

    IgG2a
  • 应用备注

    Approved: Flo, Func, IHC (25 μg/mL), Neut

    Usage: Flow cytometry, Immunohistochemistry, Neutralization of Bioactivity. Flow Cytometry-Dilute this antibody to 25 μg/mL and add 10 μL of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200 μL. The binding of unlabeled monoclonal antibodies may be visualized by adding 10 μL of a 25 μg/mL stock solution of a secondary developing reagent such as goat anti-rat IgG conjugated to a fluorochrome. Immunohistochemistry: 25 μg/mL with appropriate secondary reagents to detect CCR7 on paraformaldehyde-fixed mouse splenocytes. Neutralization of Murine CCR7 Bioactivity: The exact concentration of antibody required to neutralize mouse CCR7 activity is dependent on the cytokine concentration, cell type, growth conditions and the type of activity studied. The Neutralization Dose50 (ND50) for this antibody is defined as that concentration of antibody required to yield one-half maximal inhibition of the cytokine activity on a responsive cell line, when that cytokine is present at a concentration just high enough to elicit a maximum response. The ND50 for this lot of anti-mouse CCR7 antibody was determined to be approximately 2-10 μg/mL in the presence of 25 ng/mL of r  mMIP-3, using the BaF/3 mCCR7 cell line in a chemoattraction assay.

    说明

    Target Species of Antibody: Mouse

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Sterile PBS

    浓度

    Lot specific

    缓冲液

    Lyophilized from PBS,  pH 7.4, 5 % trehalose

    注意事项

    Avoid freeze-thaw cycles.

    储存条件

    4 °C,-20 °C

    储存方法

    Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
  • 抗原

    CCR7 (Chemokine (C-C Motif) Receptor 7 (CCR7))

    别名

    CCR7

    背景

    Name/Gene ID: CCR7
    Subfamily: Chemokine
    Family: GPCR

    Synonyms: CCR7, BLR2, Chemokine (C-C) receptor 7, CD197, C-C CKR-7, CMKBR7, CC chemokine receptor 7, CCR-7, CD197 antigen, CDw197, EBI1, EVI1, MIP-3 beta receptor, C-c chemokine receptor 7, C-C chemokine receptor type 7, CC-CKR-7, Chemokine c-c motif receptor 7

    基因ID

    1236

    UniProt

    P32248

    途径

    Regulation of Actin Filament Polymerization, Positive Regulation of Immune Effector Process
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