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CD52 抗体

This anti-CD52 antibody is a 大鼠 单克隆 antibody detecting CD52 in FACS, WB, IHC (p), IHC (fro), CyTox, IP 和 EIA. Suitable for 人 和 猴.
产品编号 ABIN320173
发货至: 中国

Quick Overview for CD52 抗体 (ABIN320173)

抗原

See all CD52 抗体
CD52

适用

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人, 猴

宿主

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大鼠

克隆类型

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单克隆

标记

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This CD52 antibody is un-conjugated

应用范围

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Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Cytotoxicity Test (CyTox), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

克隆位点

YTH34-5
  • 特异性

    SM2135LE reacts with the Human CD52 antigen, also known as CAMPATH-1. Humanised versions of CAMPATH-1 specific antibodies are currently in clinical trials for the treatment of a range of lymphoid malignancies.

    纯化方法

    Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant

    内毒素水平

    Low Endotoxin

    亚型

    IgG2b
  • 应用备注

    Flow Cytometry (1/50-1/100): Use 10 μL to label 1 x 10^6 cells in 100 μL. Immunohistochemistry (Frozen/Paraffin/Resin): This product does not require protein digestion pretreatment of paraffin section. However enhanced results may be seen using Trypsin protein digestion (2). ELISA. Western Blotting. Immunoprecipitation. Cytotoxic Assays (50 μg/mL): Use human serum as complement source.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 浓度

    1.0 mg/mL

    缓冲液

    PBS without preservatives or stabilizers.

    储存液

    Without preservative

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C/-20 °C

    储存方法

    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • 抗原

    CD52

    别名

    CD52 / CAMPATH-1

    背景

    The CD52 antigen is a remarkably small peptide that is heavily glycosylated, and attached to the cell surface membrane via a GPI link. The apparent molecular mass of the antigen on SDS-PAGE is 25-29kD. CD52 is expressed at high density by lymphocytes, monocytes, eosinophils, thymocytes and macrophages. It is expressed by most lymphoid derived malignancies, although expression on myeloma cells is variable.Synonyms: CDw52, Cambridge pathology 1 antigen, Epididymal secretory protein E5, HE5

    基因ID

    1043

    NCBI登录号

    NP_001794

    UniProt

    P31358
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