CD52 抗体
Quick Overview for CD52 抗体 (ABIN320173)
抗原
See all CD52 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- SM2135LE reacts with the Human CD52 antigen, also known as CAMPATH-1. Humanised versions of CAMPATH-1 specific antibodies are currently in clinical trials for the treatment of a range of lymphoid malignancies.
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纯化方法
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
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内毒素水平
- Low Endotoxin
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亚型
- IgG2b
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应用备注
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Flow Cytometry (1/50-1/100): Use 10 μL to label 1 x 10^6 cells in 100 μL. Immunohistochemistry (Frozen/Paraffin/Resin): This product does not require protein digestion pretreatment of paraffin section. However enhanced results may be seen using Trypsin protein digestion (2). ELISA. Western Blotting. Immunoprecipitation. Cytotoxic Assays (50 μg/mL): Use human serum as complement source.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- PBS without preservatives or stabilizers.
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储存液
- Without preservative
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- CD52
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别名
- CD52 / CAMPATH-1
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背景
- The CD52 antigen is a remarkably small peptide that is heavily glycosylated, and attached to the cell surface membrane via a GPI link. The apparent molecular mass of the antigen on SDS-PAGE is 25-29kD. CD52 is expressed at high density by lymphocytes, monocytes, eosinophils, thymocytes and macrophages. It is expressed by most lymphoid derived malignancies, although expression on myeloma cells is variable.Synonyms: CDw52, Cambridge pathology 1 antigen, Epididymal secretory protein E5, HE5
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基因ID
- 1043
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NCBI登录号
- NP_001794
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UniProt
- P31358
抗原
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