CD52 抗体
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- 抗原 See all CD52 抗体
- CD52
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适用
- 人, 猴
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宿主
- 大鼠
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克隆类型
- 单克隆
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标记
- This CD52 antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Cytotoxicity Test (CyTox), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)
- 特异性
- SM2135LE reacts with the Human CD52 antigen, also known as CAMPATH-1. Humanised versions of CAMPATH-1 specific antibodies are currently in clinical trials for the treatment of a range of lymphoid malignancies.
- 纯化方法
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- 内毒素水平
- Low Endotoxin
- 克隆位点
- YTH34-5
- 亚型
- IgG2b
- Top Product
- Discover our top product CD52 Primary Antibody
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- 应用备注
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Flow Cytometry (1/50-1/100): Use 10 μL to label 1 x 10^6 cells in 100 μL. Immunohistochemistry (Frozen/Paraffin/Resin): This product does not require protein digestion pretreatment of paraffin section. However enhanced results may be seen using Trypsin protein digestion (2). ELISA. Western Blotting. Immunoprecipitation. Cytotoxic Assays (50 μg/mL): Use human serum as complement source.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- PBS without preservatives or stabilizers.
- 储存液
- Without preservative
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- 抗原
- CD52
- 别名
- CD52 / CAMPATH-1 (CD52 产品)
- 背景
- The CD52 antigen is a remarkably small peptide that is heavily glycosylated, and attached to the cell surface membrane via a GPI link. The apparent molecular mass of the antigen on SDS-PAGE is 25-29kD. CD52 is expressed at high density by lymphocytes, monocytes, eosinophils, thymocytes and macrophages. It is expressed by most lymphoid derived malignancies, although expression on myeloma cells is variable.Synonyms: CDw52, Cambridge pathology 1 antigen, Epididymal secretory protein E5, HE5
- 基因ID
- 1043
- NCBI登录号
- NP_001794
- UniProt
- P31358
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