(2 references)-
- 抗原
- Gena
- 适用
- 小鼠
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宿主
- 大鼠
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克隆类型
- 单克隆
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标记
- 非结合性
- 应用范围
- Immunohistochemistry (IHC), Western Blotting (WB)
- 产品特性
- This product was purified from serum-free culture medium of the hybridoma clone TRA98 by proprietary chromatography processes under mild conditions.
- 纯化方法
- Purified
- 克隆位点
- TRA98
- 亚型
- IgG2a
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anti-Gena antibody
适用: 小鼠 IHC, WB 宿主: 大鼠 Monoclonal TRA98 unconjugated
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- 应用备注
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1) Western blotting
2) Immunohistochemical staining - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- PBS with 50 % Glycerol
- 储存液
- Azide free
- 储存条件
- -20 °C
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: "Regulation of proliferation and differentiation in spermatogonial stem cells: the role of c-kit and its ligand SCF." in: Development (Cambridge, England), Vol. 127, Issue 10, pp. 2125-31, (2000) (PubMed).
: "A germ cell-specific nuclear antigen recognized by a monoclonal antibody raised against mouse testicular germ cells." in: International journal of andrology, Vol. 20, Issue 6, pp. 361-6, (1998) (PubMed).
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: "Regulation of proliferation and differentiation in spermatogonial stem cells: the role of c-kit and its ligand SCF." in: Development (Cambridge, England), Vol. 127, Issue 10, pp. 2125-31, (2000) (PubMed).
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- 抗原
- Gena
- 背景
- A monoclonal antibody TRA98 recognizes a mouse testicular germ cell-specific antigen. In adult tissues, bands of 60-100 kDa proteins are detected only in the testis by Western blotting analysis with TRA98. The signals are observed in male and female embryos after embryonic day 12.5. The signal in male is detected during development of germ cells and also after birth, but the signal in female disappears by 5 days after birth. The antigen is localized only in the nuclear fraction of testicular germ cells and this antibody can be used for immunohistochemical staining of testicular germ cells. The hybridoma producing TRA98 was obtained by immunization of a rat with the cell lysate of adult mouse testis by the group of Prof. Yoshitake Nishimune of Osaka University.
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