SLA (Haploid Sperm Cell-Specific Antigen) 抗体
There are 3+ publications for this product available. The 大鼠 单克隆 anti-SLA (Haploid Sperm Cell-Specific Antigen) antibody is suitable to detect SLA (Haploid Sperm Cell-Specific Antigen) in samples from 小鼠 和 大鼠. It has been validated for IEM, IHC 和 WB.
(3 references)
产品编号 ABIN3201019
发货至:
中国
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中国
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北京 101111
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Quick Overview for SLA (Haploid Sperm Cell-Specific Antigen) 抗体 (ABIN3201019)
抗原
SLA (Haploid Sperm Cell-Specific Antigen)
适用
小鼠, 大鼠
宿主
大鼠
克隆类型
单克隆
应用范围
Immunoelectron Microscopy (IEM), Immunohistochemistry (IHC), Western Blotting (WB)
克隆位点
TRA54
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交叉反应 （详细）
- Not tested in other species.
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产品特性
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The antibody was purified from the serum-free culture medium of the hybridoma clone TRA54 by proprietary chromatography processes under mild conditions.
This antibody can be used for immunohistochemical staining of testicular tissues. The hybridoma producing TRA54 was obtained from the immunaization of a rat with cell lysates of adult mouse testes by the group of Prof. Yoshitake Nishimune of Osaka University. -
纯化方法
- Purified
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亚型
- IgM
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应用备注
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1. Western blotting: ~1 g/mL
2. Immunohistochemical staining
3. Immunoelectron microscopy
Not tested in other applications -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- PBS with 50 % Glycerol
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储存液
- Azide free
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储存条件
- -20 °C
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: "Identification and characterization of an antigen recognized by monoclonal antibody TRA 54 in mouse epididymis and vas deferens." in: Journal of andrology, Vol. 25, Issue 6, pp. 914-21, (2004) (PubMed).
: "Intercellular organelle traffic through cytoplasmic bridges in early spermatids of the rat: mechanisms of haploid gene product sharing." in: Molecular biology of the cell, Vol. 14, Issue 7, pp. 2768-80, (2003) (PubMed).
: "Characterization and expression of a stage specific antigen by monoclonal antibody TRA 54 in testicular germ cells." in: International journal of andrology, Vol. 21, Issue 1, pp. 34-40, (1998) (PubMed).
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: "Identification and characterization of an antigen recognized by monoclonal antibody TRA 54 in mouse epididymis and vas deferens." in: Journal of andrology, Vol. 25, Issue 6, pp. 914-21, (2004) (PubMed).
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- SLA (Haploid Sperm Cell-Specific Antigen)
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背景
- Many transcripts for proteins specifically expressed in haploid germ cells are synthesized in the spermatogenesis. A monoclonal antibody TRA54 recognizes specific organelles in germ cell cytoplasm from spermatocytes to spermatids. Western blots using TRA54 revealed broad bands with 85 kD, 190 kD and >200 kDa in testis probably due to alternative splicing and/or post-translational modification. The molecular size diversity is due to the difference in glycosilation.
抗原
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