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Flavivirus E protein 抗体

适用: 人, 小鼠 IF, WB 宿主: 小鼠 Monoclonal 4G2 unconjugated
产品编号 ABIN3200993
发货至: 中国
  • 抗原
    Flavivirus E protein
    适用
    人, 小鼠
    宿主
    小鼠
    克隆类型
    单克隆
    应用范围
    Immunofluorescence (IF), Western Blotting (WB)
    特异性
    E protein of Flavivirus
    产品特性
    Mouse monclonal generated against flavivirus envelope proteins.  Recognizes Dengue virus, West Nile Virus, Japanese Encephalitis and  Zika virus. It binds to domain II of protein E (fusion loop).  
    纯化方法
    Purified from hybridoma supernatant via Protein G
    免疫原
    Dengue Virus
    克隆位点
    4G2
    亚型
    IgG2a
  • 应用备注
    0.1-1.0 µg/mL
    限制
    仅限研究用
  • 生效 #101139 (Immunocytochemistry)
    '独立验证'标志
    by
    Division of Clinical Pharmacology, LMU, Munich
    No.
    #101139
    日期
    2017.11.07
    抗原
    Flavivirus E protein
    Lot Number
    Method validated
    Immunocytochemistry
    Positive Control
    1205Lu human melanoma cells infected with yellow fever vaccine strain YF-17D
    Negative Control
    Uninfected 1205Lu cells; infected cells without primary antibody
    Notes
    ABIN3200993 specifically labels the targeted antigen in 1205Lu melanoma cells in ICC. No signal was detected in uninfected negative control tissue and the secondary antibody only control.
    '独立验证'标志
    Validation Images
    Full Methods
    Primary Antibody
    ABIN3200993
    Secondary Antibody
    goat-anti mouse AF647 conjugated antibody (Invitrogen, A21235, lot 1764240)
    Full Protocol
    • Grow cells in DMEM supplemented with 10% FCS, 1% L-Glutamin, 1% Pen/Strep (Gibco) to 70% confluency on coverslips coated with Poly L-Lysin (Sigma, P8920).
    • Infect cells with yellow fever vaccine strain YF-17D at 2MOI.
    • Grow cells on coverslips for 48h.
    • Fix cells on coverslips in 4% PFA for 30min at RT.
    • Wash cells 2x with PBS.
    • Permeabilize cells in PBScontaining 0.1% Triton-X 100 (PBST) for 1h at RT.
    • Wash cells 2x with PBS.
    • Block non-specific binding with in PBST containing 10% normal goat serum for 2h at RT.
    • Incubate slides with primary mouse anti-Flavivirus E protein antibody (antibodies-online, ABIN3200993) diluted 1:200 and rabbit-anti ER antibody (abcam, ab176333, GR265198-3) diluted 1:200 in PBST with 10% NGS ON at 4°C.
    • Include wells without primary antibody as negative controls.
    • Rinse slides 3x with PBST.
    • Incubate slides with secondary goat-anti mouse AF647 conjugatedantibody (Invitrogen, A21235, lot 1764240) diluted 1:1000 and goat-anti rabbit AF488 conjugated antibody (Invitrogen, A-11008, lot 84B2-1) diluted 1:1000 in PBST with 3% NGS for 1h at RT.
    • Rinse slide 3x with PBST.
    • Nuclear counterstain with DAPI diluted in PBS (Sigma, 10236276001).
    • Wash cells 2x with PBS.
    • Mount coverslips on glass slides in Mowiol antifade reagent (Calbiochem, 475904).
    • Image acquisition with Leica SP5 II confocal imaging system, 20x magnification, 1024x1024 resolution.
    Experimental Notes
  • 状态
    Liquid
    浓度
    1 mg/mL
    缓冲液
    PBS pH 7.4
    储存条件
    -20 °C
    储存方法
    Heat stable , shipped at ambient  temp  Upon delivery aliquot and store in fridge , longterm storage at  -20°C.
  • Tang, Hammack, Ogden, Wen, Qian, Li, Yao, Shin, Zhang, Lee, Christian, Didier, Jin, Song, Ming: "Zika Virus Infects Human Cortical Neural Progenitors and Attenuates Their Growth." in: Cell stem cell, Vol. 18, Issue 5, pp. 587-90, (2017) (PubMed).

  • 抗原
    Flavivirus E protein
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