This antibody recognises VCAM-1. This antibody inhibits cellular adhesion mediated by VCAM-1. Removal of Sodium Azide is recommended prior to use in functional assays.
纯化方法
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Flow Cytometry (1/10-1/50). ELISA. Immunoprecipitation. Immunohistochemistry on Frozen Sections. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
1.0 mg/mL
缓冲液
PBS containing 0.09 % Sodium Azide as preservative and 0.1 % BSA as stabilizer.
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
VCAM1 is a 110kD molecule whose ligand is VLA4. VCAM1 is important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the integrins alpha4 beta1 (beta 1 integrin VLA4) and alpha4 beta7 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation. VCAM1 is also expressed by several non endothelial cell types including some macrophages, follicular dendritic cells and bone marrow, stromal cells.Synonyms: INCAM-100, L1CAM, V-CAM 1, VCAM-1, Vascular cell adhesion protein 1