ITGA2 抗体
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- 抗原 See all ITGA2 抗体
- ITGA2 (Integrin alpha 2 (ITGA2))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This ITGA2 antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)
- 特异性
- This antibody is identified as capable of immunoprecipitating a non-reducible alpha subunit from I125 surface labelled cell line extract. It is confirmed as specific to the alpha 2 subunit by relative expression of antigen on various cell lines by FACS, and its recognition of affinity purified alpha 2 beta 1 in dot blots.
- 无交叉反应
- 大鼠
- 交叉反应 (详细)
- Species reactivity (tested):Human.
- 纯化方法
- Affinity Chromatography on Protein G
- 免疫原
- Purified human beta1 preparation from HT1080 fibrosarcoma cell extract. Spleen cells from immunised BALB/c mice were fused with cells of the X63/Ag8.653 mouse myeloma cell line.
- 克隆位点
- 16B4
- 亚型
- IgG1
- Top Product
- Discover our top product ITGA2 Primary Antibody
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- 应用备注
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Flow Cytometry: 30 μg/mL, Immunoprecipitation: 25-50 μg/mL. Immunohistochemistry on Frozen Sections: 30-40 μg/mL. ELISA: 10 μg/mL. Western Blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- 抗原
- ITGA2 (Integrin alpha 2 (ITGA2))
- 别名
- CD49b / ITGA2 (ITGA2 产品)
- 背景
- CD49b, a member of the integrin family of extracellular matrix and cell adhesion molecules, is the integrin alpha-2 chain. CD49b associates noncovalently with CD29, the integrin beta-1 subunit, to form the heterodimer integrin a2b1 that binds laminin and collagen. It is expressed on B and T lymphocytes, NK cells, monocytes, platelets, fibroblasts and long-term cultured T cells. CD49b/CD29 mediates the Mg++ dependent adhesion of platelets to collagen. It may also be utilized by fibroblasts for the reorganization of the collagen matrix during would healing.Synonyms: CD49 antigen-like family member B, Collagen Receptor, GPIa, ITGA-2, Integrin alpha-2, Platelet membrane glycoprotein Ia, VLA-2 alpha, VLA2
- 基因ID
- 3673
- NCBI登录号
- NP_002194
- UniProt
- P17301
- 途径
- CXCR4-mediated Signaling Events, Smooth Muscle Cell Migration, Integrin Complex
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