AGO4 抗体
-
- 抗原 See all AGO4 (EIF2C4) 抗体
- AGO4 (EIF2C4) (Eukaryotic Translation Initiation Factor 2C, 4 (EIF2C4))
-
适用
- Arabidopsis thaliana, Hyacinthus orientalis (Common hyacinth)
-
宿主
- 兔
-
克隆类型
- 多克隆
-
标记
- This AGO4 antibody is un-conjugated
-
应用范围
- Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Co-Immunoprecipitation ( Co-IP)
- 交叉反应 （详细）
- Not reactive in: Brassica oleracea, Hordeum vulgare, Solanum lycopersicum, Zea mays, Vigna angularis
- 预测反应
- Arabidopsis thaliana
- 产品特性
-
Expected / apparent Molecular Weight of the Antigene: 103 kDa
Antibody concentration is 0.4 mg/mL in 50% glycerol, PBS, 0.005% thimerosal,0.05% BSA, threfore the antibody sample will not freeze in -20°C.binds enDog (Canine)enous siRNAs, preference for 24nt siRNAs with 5' A. - 纯化方法
- serum
- 免疫原
- KLH-conjugated synthetic peptide chosen from Arabidopsis thaliana AGO4 sequence UniProt: Q9ZVD5 , TAIR: At2g27040.
- Top Product
- Discover our top product EIF2C4 Primary Antibody
-
anti-Eukaryotic Translation Initiation Factor 2C, 4 (EIF2C4) (AA 716-861) antibody
EIF2C4 适用: 人 ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 2C, 4 (EIF2C4) antibodyEIF2C4 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 2C, 4 (EIF2C4) antibodyEIF2C4 适用: 人, 小鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 2C, 4 (EIF2C4) (AA 1-100) antibodyEIF2C4 适用: 人 ELISA 宿主: 小鼠 Monoclonal 4G3 unconjugated
anti-Eukaryotic Translation Initiation Factor 2C, 4 (EIF2C4) (N-Term) antibodyEIF2C4 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 2C, 4 (EIF2C4) (AA 611-660) antibodyEIF2C4 适用: 人, 大鼠, 兔, 犬, 豚鼠, 猴, Pig WB 宿主: 兔 Polyclonal unconjugated
-
- 应用备注
- 1: 4000 (WB), 5 μg of antibody per 1 gram of a fresh tissue (IP), 1: 100 (ICC)
- 说明
-
AGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest. Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure. Use a 6 % gel for protein separation, which is run longer to avoid a cross-reactivity at ca. 40 kDa.Note that the AGO4 antibody reacts with the NEB prestained protein marker.
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 溶解方式
- For reconstitution add 50 μL of sterile water.
- 缓冲液
- Antibody concentration is 0.4 mg/mL in 50 % glycerol, PBS, 0.005 % thimerosal,0.05 % BSA. Please, add 125 µl of deionized water and let it stand in 4 °C overnight to mix. Afterwards warm the antibody sample up to RT and allow theglycerol to dissolve well. Mak
- 储存液
- Thimerosal (Merthiolate)
- 注意事项
- This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
- 储存条件
- -20 °C
- 储存方法
- Store at -20°C, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur adhering to the cap or sides of the tubes.
-
- 抗原
- AGO4 (EIF2C4) (Eukaryotic Translation Initiation Factor 2C, 4 (EIF2C4))
- 别名
- AGO4 (EIF2C4 产品)
- 别名
- EIF2C4 antibody, ago4 antibody, Argonaute4 antibody, argonaute-4 antibody, eif2c4 antibody, wu:fd14f04 antibody, ARGONAUTE 4 antibody, OCP11 antibody, OVEREXPRESSOR OF CATIONIC PEROXIDASE 11 antibody, T20P8.9 antibody, T20P8_9 antibody, Eif2c4 antibody, 5730550L01Rik antibody, AI481660 antibody, argonaute 4, RISC catalytic component antibody, argonaute 1, RISC catalytic component antibody, argonaute RISC catalytic component 4 antibody, Argonaute family protein antibody, argonaute 4, RISC catalytic component L homeolog antibody, argonaute RISC catalytic subunit 4 antibody, AGO4 antibody, ago1 antibody, ago4 antibody, Ago4 antibody, ago4.L antibody
- 背景
-
AGI Code: At2g27040
AGO4 (Argonaute 4) belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC). This protein complex is responsible for the gene silencing (RNAi). - 分子量
- 103 kDa
- UniProt
- Q9ZVD5
- 途径
- Fc-epsilon Receptor Signaling Pathway, Regulatory RNA Pathways, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Cellular Glucan Metabolic Process
-
