AGO4 抗体
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Quick Overview for AGO4 抗体 (ABIN3197495)
抗原
See all AGO4 (EIF2C4) 抗体适用
宿主
克隆类型
标记
应用范围
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原理
- Anti-AGO4 | Argonaute 4
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交叉反应 （详细）
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Arabidopsis thaliana, Hyacinthus orientalis
Not reactive in: Brassica oleracea, Fragaria x ananassa, Hordeum vulgare, Poplar sp.,Solanum lycopersicum, Vigna angularis, Zea mays -
预测反应
- Arabidopsis thaliana
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纯化方法
- Immunogen affinity purified serum
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免疫原
- KLH-conjugated synthetic peptide chosen from Arabidopsis thaliana AGO4 sequence UniProt: Q9ZVD5
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anti-Eukaryotic Translation Initiation Factor 2C, 4 (EIF2C4) (AA 716-861) antibody
EIF2C4 适用: 人 ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 2C, 4 (EIF2C4) antibodyEIF2C4 适用: 人, 小鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 2C, 4 (EIF2C4) (AA 1-100) antibodyEIF2C4 适用: 人 ELISA 宿主: 小鼠 Monoclonal 4G3 unconjugated
anti-Eukaryotic Translation Initiation Factor 2C, 4 (EIF2C4) (N-Term) antibodyEIF2C4 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 2C, 4 (EIF2C4) (AA 611-660) antibodyEIF2C4 适用: 人, 大鼠, 犬, 豚鼠, 兔, 猴, Pig WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- 1 : 100 (ICC), 5 μg of antibody per 1 gram of a fresh tissue (IP),1 : 2000-1 : 5000 (WB)
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说明
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AGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest. Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure. Use a 6 % gel for protein separation, which is run longer to avoid a cross-reactivity at ca. 40 kDa.Note that the AGO4 antibody reacts with the NEB prestained protein marker.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- For reconstitution add 50 μL of sterile water
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缓冲液
- Immunogen affinity purified serum in PBS pH 7.4.
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储存条件
- -20 °C
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储存方法
- Store lyophilized/reconstituted at -20°C, Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur adhering to the cap or sides of the tube.
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- AGO4 (EIF2C4) (Eukaryotic Translation Initiation Factor 2C, 4 (EIF2C4))
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别名
- AGO4
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背景
- AGO4 (Argonaute 4) belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC). This protein complex is responsible for the gene silencing (RNAi).
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分子量
- 103 kDa
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基因ID
- 817246
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UniProt
- Q9ZVD5
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途径
- Fc-epsilon Receptor Signaling Pathway, Regulatory RNA Pathways, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Cellular Glucan Metabolic Process
抗原
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