AOX1/AOX2 抗体 (C-Term)
Cited in 7+ publications.
The 兔 多克隆 anti-AOX1/AOX2 antibody (ABIN3197483) specifically detects AOX1/AOX2 in IL 和 WB.
The antibody is reactive with Physcomytrium Patens, Zea mays, Triticum aestivum, Tomato, Symplocarpus renifolius, Soybean, Rosa hybrida, Robinia pseudoacacia, Rape, Potato, Poa annua, Picea abies, Arabidopsis thaliana, Pea, Oryza sativa, Nicotiana tabacum, Lupinus luteus, Kandelia candel, Eriophorum vaginatum, Copepod (Tigriopus), Cabbage, Betula nana, Beet 和 Barley samples.
2 images
(7 references)
产品编号 ABIN3197483
发货至:
中国
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Contact Info
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Quick Overview for AOX1/AOX2 抗体 (C-Term) (ABIN3197483)
抗原
AOX1/AOX2
(Alternative Oxidase 1/2 (AOX1/AOX2))
适用
Physcomytrium Patens, Zea mays, Triticum aestivum, Tomato, Symplocarpus renifolius, Soybean, Rosa hybrida, Robinia pseudoacacia, Rape, Potato, Poa annua, Picea abies, Arabidopsis thaliana, Pea, Oryza sativa, Nicotiana tabacum, Lupinus luteus, Kandelia candel, Eriophorum vaginatum, Copepod (Tigriopus), Cabbage, Betula nana, Beet, Barley
宿主
兔
克隆类型
多克隆
应用范围
Immunolocalization (IL), Western Blotting (WB)
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抗原表位
- C-Term
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原理
- Anti-AOX1/2 | Plant alternative oxidase 1 and 2
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交叉反应 （详细）
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Arabidopsis thaliana, Betula nana, Beta vulgaris, Brassica napus, Brassica oleracea, Kandelia candel, Eriphorum vaginatum, Glycine max, Hordeum vulgare, Lupinus luteus, Nicotiana tabacum, Oryza sativa, Picea abies, Pisum sativum, Poa annua, Robinia pseudoacacia, Rosa hybrida, Solanum lycopersicum, Solanum tuberosum, Symplocarpus renifolius, Physcomitrium patens, Tigriopus californicus, Triticum aestivum, Zea mays
Not reactive in: Candidia albicans, Chlamydomonas reinhardtii (use an antibody to algal AOX1, AS06 152), Stomolophus sp2 -
预测反应
- Aegilops tauschii,Brachypodium distachyon,Capsella rubella,Citrus sinensis,Citrus clementina,Corylus heterophylla,Crocus sativus,Cucumis sativus,Daucus carota,Hypericum perforatum,Lotus japonicus,Malus x domestica,Medicago truncatula,Medicago sativa,Mangifera indica,Naegleria gruberi (amoeba),Nelumbo nucifera,Nicotiana benthamiana,Oryza brachyantha,Populus tremula,Picea sitchensis,Pinus pinaster,Pyrus communis,Saccharum officinarum,Sauromatum venosum,Sorghum bicolor,Selaginella moellendorffii,Tetrahymena thermophila,Vigna radiata,Vigna unguiculata,Vitis vinifera
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纯化方法
- Serum
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免疫原
- KLH-conjugated synthetic peptide derived from fully conserved C-terminal consensus motif from plant AOX isoforms including Arabidopsis thaliana AOX1A. UniProt: Q39219
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应用备注
- 1 : 750 (IL), 1 : 1000 for 10-20 μg of mitochondrial protein/lane detection (WB)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- For reconstitution, add 50 μL of sterile water
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缓冲液
- Serum
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注意事项
- Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube
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储存条件
- -20 °C
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储存方法
- Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
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: "Overexpression of UCP1 in tobacco induces mitochondrial biogenesis and amplifies a broad stress response." in: BMC plant biology, Vol. 14, pp. 144, (2014) (PubMed).
: "Low temperature storage affects the ascorbic acid metabolism of cherry tomato fruits." in: Plant physiology and biochemistry : PPB / Société française de physiologie végétale, Vol. 84, pp. 149-57, (2014) (PubMed).
: "Inhibition of the electron transport strongly affects transcription and transcript levels in Arabidopsis mitochondria." in: Mitochondrion, Vol. 19 Pt B, pp. 222-30, (2014) (PubMed).
: "A lack of mitochondrial alternative oxidase compromises capacity to recover from severe drought stress." in: Physiologia plantarum, (2013) (PubMed).
: "Respiratory flexibility and efficiency are affected by simulated global change in Arctic plants." in: The New phytologist, Vol. 197, Issue 4, pp. 1161-72, (2013) (PubMed).
: "Simplified enrichment of plasma membrane proteins for proteomic analyses in Arabidopsis thaliana." in: Proteomics, Vol. 11, Issue 9, pp. 1780-8, (2011) (PubMed).
: "Nitric oxide increases mitochondrial respiration in a cGMP-dependent manner in the callus from Arabidopsis thaliana." in: Nitric oxide : biology and chemistry / official journal of the Nitric Oxide Society, (2010) (PubMed).
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: "Overexpression of UCP1 in tobacco induces mitochondrial biogenesis and amplifies a broad stress response." in: BMC plant biology, Vol. 14, pp. 144, (2014) (PubMed).
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- AOX1/AOX2 (Alternative Oxidase 1/2 (AOX1/AOX2))
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别名
- Plant alternative oxidase 1 and 2 (AOX1/2)
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背景
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Background: Alternative oxidases (AOX) are quinol oxidases located in the inner mitochondrial membrane of plants. They function as terminal oxidases in the alternate electron transport pathway, oxidizing ubiquinone to reduce oxygen to water.
Additional information: Mitochondrion inner membrane marker. Possibly in the inner surface of the inner mitochondrial membrane.Protocol for a plant mitochondria preparation can be found here. In protein samples which are older than few months AOX enzyme can undergo intensive dimerization. Such preparations should not be used to work with this antibody.
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分子量
- 36-40 | 36-40 for Arabidopsis thaliana
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基因ID
- 821806
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UniProt
- Q39219, O22049, Q7XBG9, Q7XT33
抗原
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