Sialoadhesin/CD169 抗体
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- 抗原 See all Sialoadhesin/CD169 (SIGLEC1) 抗体
- Sialoadhesin/CD169 (SIGLEC1) (Sialic Acid Binding Ig-Like Lectin 1, Sialoadhesin (SIGLEC1))
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适用
- 小鼠
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宿主
- 大鼠
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克隆类型
- 单克隆
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标记
- This Sialoadhesin/CD169 antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF)
- 特异性
- This antibody is specific for CD169, also known as Sialoadhesin. It reacts with a subpopulation of mature resident tissue macrophages. No reactivity is seen with dendritic cells, peritoneal resident macrophages, peritoneal exudate cells or blood cells. Distinct macrophage subpopulations of lymphoid organs express the antigen. In the spleen, they are localised at the marginal sinus forming a ring around the periarteriolar lymphocyte sheath and follicular areas at the inner side of marginal zones. In lymph nodes, they are localised in the sinusoids and medullary cords but not within follicular areas or paracortex. In Peyer's patches they are localised in the interfollicular areas at the serosal side. Kupffer cells in the liver can be clearly stained by MOMA-1, although antigen expression is weaker than that seen in splenic macrophages. No MOMA-1 positive macrophages were found in the thymus. Reactivity was also negative in following organs tested so far (kidney, brain, skin). In non-lymphoid organs, the antigen is only found on a macrophage subpopulation in the lamina propria of the villi of the small intestine.
- 纯化方法
- Tissue Culture Supernatant containing 0.2M Tris/HCl pH7.4 and 5-10% foetal calf serum
- 免疫原
- Stromal (reticular) elements from spleen.
- 克隆位点
- MOMA-1
- 亚型
- IgG2a
- Top Product
- Discover our top product SIGLEC1 Primary Antibody
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anti-Sialic Acid Binding Ig-Like Lectin 1, Sialoadhesin (SIGLEC1) (AA 851-950) antibody
SIGLEC1 适用: 人, 大鼠 FACS, ELISA, IHC (fro), IF (cc), IF (p), ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Sialic Acid Binding Ig-Like Lectin 1, Sialoadhesin (SIGLEC1) (AA 20-197) antibodySIGLEC1 适用: 人 FACS, ELISA, ICC 宿主: 小鼠 Monoclonal 2A4D1 unconjugated
anti-Sialic Acid Binding Ig-Like Lectin 1, Sialoadhesin (SIGLEC1) (AA 572-697) antibodySIGLEC1 适用: 人 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Sialic Acid Binding Ig-Like Lectin 1, Sialoadhesin (SIGLEC1) antibody (APC)SIGLEC1 适用: 人 FACS 宿主: 小鼠 Monoclonal 7-239 APC
anti-Sialic Acid Binding Ig-Like Lectin 1, Sialoadhesin (SIGLEC1) antibodySIGLEC1 适用: 大鼠 FACS, IP, IHC (fro), IF 宿主: 小鼠 Monoclonal ED3 unconjugated
anti-Sialic Acid Binding Ig-Like Lectin 1, Sialoadhesin (SIGLEC1) antibodySIGLEC1 适用: 人 FACS, WB, IP, IHC (fro) 宿主: 小鼠 Monoclonal 7-239 unconjugated
anti-Sialic Acid Binding Ig-Like Lectin 1, Sialoadhesin (SIGLEC1) antibody (PE)SIGLEC1 适用: 人 FACS 宿主: 小鼠 Monoclonal 7-239 PE
anti-Sialic Acid Binding Ig-Like Lectin 1, Sialoadhesin (SIGLEC1) (AA 851-950) antibody (AbBy Fluor® 647)SIGLEC1 适用: 人, 大鼠 FACS, IF (cc), IF (p) 宿主: 兔 Polyclonal AbBy Fluor® 647
anti-Sialic Acid Binding Ig-Like Lectin 1, Sialoadhesin (SIGLEC1) (Internal Region) antibodySIGLEC1 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Sialic Acid Binding Ig-Like Lectin 1, Sialoadhesin (SIGLEC1) (N-Term) antibodySIGLEC1 适用: 人 FACS, WB, IHC, IF, StM 宿主: 小鼠 Monoclonal HSn 7D2 unconjugated
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- 应用备注
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Flow Cytometry: Use 10 μL of 1/10-1/50 diluted antibody to label 10^6 cells in 100 μL. Immunohistochemistry on Frozen Sections (1/10).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 0.2 M Tris/HCl, pH 7.4 and 5-10 % Foetal Calf Serum, 0.09 % Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- 抗原
- Sialoadhesin/CD169 (SIGLEC1) (Sialic Acid Binding Ig-Like Lectin 1, Sialoadhesin (SIGLEC1))
- 别名
- CD169 / SIGLEC1 (SIGLEC1 产品)
- 背景
- The sialoadhesin family has 4 members: CD22, a B cell specific marker, myelin associated glycoprotein (MAG), which is expressed on oligodendrocytes and Schwann cells, CD33, a myeloid differentiation antigen, and sialoadhesin, which is expressed only by a subpopulation of tissue macrophages. Involved in cell-cell interactions, sialoadhesin is structurally related to the 3 other listed members of the sialoadhesin family. CD169 is a sialic acid binding site of sialoadhesin. CD169 is a macrophage receptor expressed on stromal macrophages in many tissues, particularly found in lymph nodes, bone marrow and spleen.Synonyms: Sialic acid-binding Ig-like lectin 1, Sialoadhesin, Siglec-1
- 基因ID
- 20612
- NCBI登录号
- NP_035556
- UniProt
- Q62230
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