Transferrin Receptor 抗体
-
- 抗原 See all Transferrin Receptor (TFRC) 抗体
- Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))
-
适用
- 小鼠
-
宿主
- 大鼠
-
克隆类型
- 单克隆
-
标记
- This Transferrin Receptor antibody is un-conjugated
-
应用范围
- Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 特异性
- This antibody reacts with the Transferrin Receptor (TFR). Expressed on all dividing cells and can distinguish resting and activated T-cells. Immunoprecipitates a disulphide linked homodimer of 90 kDa by SDS PAGE. The antibody inhibits cell proliferation and the mixed lymphocyte response in vitro. Blocks the binding of R17 217.1.3. and R17 208.2 anti-TFR monoclonal antibodies (1,2).
- 纯化方法
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- 免疫原
- Mouse Concanavilin A activated spleen cells. Spleen cells from an immunised DA rat were fused with cells of the Y3/Ag1.2.3 rat myeloma cell line.
- 克隆位点
- YTA74-4
- 亚型
- IgG2a
- Top Product
- Discover our top product TFRC Primary Antibody
-
-
- 应用备注
-
Flow Cytometry: Use 10 μL of 1/50-1/100 diluted antibody to label 10^6 cells in 100 μL. Immunoprecipitation. Immunohistochemistry on Frozen Sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
-
- 浓度
- 1.0 mg/mL
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
-
- 抗原
- Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))
- 别名
- CD71 / TFRC (TFRC 产品)
- 背景
- CD71 antigen expression is low on normal resting lymphocytes and is expressed on all cells upon activation. The transferrin receptor is essential for iron transport into proliferating cells, such as mitogen-activated and alloantigen-activated lymphoblasts. The transferrin receptor is also present on early erythroid cells but is lost as reticulocytes differentiate into mature erythrocytes.Synonyms: TfR1, Transferrin receptor protein 1, p90
- 基因ID
- 22042
- NCBI登录号
- NP_035768
- UniProt
- Q62351
- 途径
- Transition Metal Ion Homeostasis
-