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EIF4G1 抗体 (Ser1232)

This anti-EIF4G1 antibody is a 兔 多克隆 antibody detecting EIF4G1 in WB, IF 和 IHC (p). Suitable for 人.
产品编号 ABIN319387
发货至: 中国

Quick Overview for EIF4G1 抗体 (Ser1232) (ABIN319387)

抗原

See all EIF4G1 抗体
EIF4G1 (Eukaryotic Translation Initiation Factor 4 Gamma, 1 (EIF4G1))

适用

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宿主

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克隆类型

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多克隆

标记

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This EIF4G1 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
  • 抗原表位

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    Ser1232

    特异性

    This antibody detects endogenous levels of total eLF4G protein.

    纯化方法

    Immunoaffinity Chromatography.

    免疫原

    The antiserum was produced against synthesized non-phosphopeptide derived from human eIF4G around the phosphorylation site of Serine 1232 (P-V-Sp-P-L).
  • 应用备注

    Western Blot (1/500-1/1000). Immunohistochemistry (1/50-1/100). Immunofluorescence (1/100-1/200).
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 缓冲液

    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Store the antibody (in aliquots) at-20 °C.
  • 抗原

    EIF4G1 (Eukaryotic Translation Initiation Factor 4 Gamma, 1 (EIF4G1))

    别名

    EIF4G1 / EIF4F

    背景

    EIF4G1 (eukaryotic translation Initiation Factor 4 Gamma 1) is a component of the protein complex eIF-4 which is involved in the recognition of the mRNA cap ATP-dependent unwinding of the 5'-terminal secondary structure and recruitment of mRNA to the ribosome. eIF4G plays a critical role in protein expression and is at the center of a complex regulatory network. Together with the cap-binding protein eIF4E, it recruits the small ribosomal subunit to the 5'-end of mRNA and promotes the assembly of a functional translation initiation complex which scans along the mRNA to the translation start codon. Human eIF4G contains three consecutive HEAT domains, as well as long unstructured regions involved in multiple protein-protein interactions. The interactions of eIF4G1 with other factors are largely unknown.Synonyms: EIF4G, EIF4GI, Eukaryotic translation initiation factor 4 gamma 1, eIF-4-gamma 1, eIF-4G 1, eIF-4G1, p220

    基因ID

    1981

    NCBI登录号

    NP_886553

    UniProt

    Q04637
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