EIF4G1 抗体 (Ser1232)
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Quick Overview for EIF4G1 抗体 (Ser1232) (ABIN319387)
抗原
See all EIF4G1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Ser1232
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特异性
- This antibody detects endogenous levels of total eLF4G protein.
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纯化方法
- Immunoaffinity Chromatography.
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免疫原
- The antiserum was produced against synthesized non-phosphopeptide derived from human eIF4G around the phosphorylation site of Serine 1232 (P-V-Sp-P-L).
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应用备注
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Western Blot (1/500-1/1000). Immunohistochemistry (1/50-1/100). Immunofluorescence (1/100-1/200).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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缓冲液
- PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Store the antibody (in aliquots) at-20 °C.
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- EIF4G1 (Eukaryotic Translation Initiation Factor 4 Gamma, 1 (EIF4G1))
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别名
- EIF4G1 / EIF4F
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背景
- EIF4G1 (eukaryotic translation Initiation Factor 4 Gamma 1) is a component of the protein complex eIF-4 which is involved in the recognition of the mRNA cap ATP-dependent unwinding of the 5'-terminal secondary structure and recruitment of mRNA to the ribosome. eIF4G plays a critical role in protein expression and is at the center of a complex regulatory network. Together with the cap-binding protein eIF4E, it recruits the small ribosomal subunit to the 5'-end of mRNA and promotes the assembly of a functional translation initiation complex which scans along the mRNA to the translation start codon. Human eIF4G contains three consecutive HEAT domains, as well as long unstructured regions involved in multiple protein-protein interactions. The interactions of eIF4G1 with other factors are largely unknown.Synonyms: EIF4G, EIF4GI, Eukaryotic translation initiation factor 4 gamma 1, eIF-4-gamma 1, eIF-4G 1, eIF-4G1, p220
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基因ID
- 1981
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NCBI登录号
- NP_886553
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UniProt
- Q04637
抗原
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