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Dynamin 1 抗体 (pSer774)

This 绵羊 多克隆 anti-Dynamin 1 antibody specifically detects Dynamin 1 in WB, IF 和 IHC (fro). The antibody is reactive with 人, 小鼠, 大鼠, 犬, Cow, 小鸡 和 猴 samples.
产品编号 ABIN319098
发货至: 中国
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Quick Overview for Dynamin 1 抗体 (pSer774) (ABIN319098)

抗原

See all Dynamin 1 (DNM1) 抗体
Dynamin 1 (DNM1)

适用

  • 106
  • 51
  • 49
  • 9
  • 8
  • 7
  • 2
  • 2
  • 2
  • 2
  • 1
人, 小鼠, 大鼠, 犬, Cow, 小鸡, 猴

宿主

  • 98
  • 9
  • 2
绵羊

克隆类型

  • 96
  • 14
多克隆

标记

  • 48
  • 6
  • 5
  • 4
  • 3
  • 3
  • 3
  • 3
  • 3
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  • 3
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
This Dynamin 1 antibody is un-conjugated

应用范围

  • 96
  • 39
  • 39
  • 34
  • 29
  • 14
  • 14
  • 13
  • 12
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))
  • 抗原表位

    • 31
    • 22
    • 15
    • 5
    • 4
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    pSer774

    特异性

    This antibody is specific for Dynamin when phosphorylated at Serine 774.

    交叉反应 (详细)

    Species reactivity (expected):Human, Monkey, Bovine, Chicken, Mouse, Dog.
    Species reactivity (tested):Rat.

    纯化方法

    Affinity Chromatography.

    免疫原

    Synthetic phosphopeptide corresponding to an amino acid sequence within Dynamin which includes phosphorylated Ser774. Genename: DNM

    亚型

    IgG
  • 应用备注

    Immunofluorescence. Immunohistochemistry on Frozen Sections. Western Blot (1/1000): This antibody detects a band of approximately 95 kDa in Rathippocampus lysates. For the detection of Phosphoproteins, Serine Phosphatase inhibitors such as 10 mMSodium Fluoride should be added to the sample buffer. Milk or other casein-basedblocking solutions are not recommended as casein is a Phosphoprotein and its use canresult in high background.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 状态

    Liquid

    缓冲液

    10 mM HEPES, pH 7.5 containing 0.09 % Sodium Azide, 0.01 % BSA and 50 % Glycerol.

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C/-20 °C

    储存方法

    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • 抗原

    Dynamin 1 (DNM1)

    别名

    Dynamin-1

    背景

    Dynamin is a phosphoprotein expressed at nerve terminals, which plays an important role in the regulation of synaptic vesicle endocytosis. Cyclin dependent protein kinase 5 (cdk5) has been shown to phosphorylate serine 774 and 778 in vivo. Dephosphorylation of these residues is essential for endocytosis of synaptic vesicles.Synonyms: DNM, DNM1

    基因ID

    140694

    NCBI登录号

    NP_542420

    UniProt

    P21575

    途径

    Toll-Like Receptors Cascades, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling
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