Dynamin 1 抗体 (pSer774)
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Quick Overview for Dynamin 1 抗体 (pSer774) (ABIN319098)
抗原
See all Dynamin 1 (DNM1) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pSer774
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特异性
- This antibody is specific for Dynamin when phosphorylated at Serine 774.
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交叉反应 (详细)
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Species reactivity (expected):Human, Monkey, Bovine, Chicken, Mouse, Dog.
Species reactivity (tested):Rat. -
纯化方法
- Affinity Chromatography.
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免疫原
- Synthetic phosphopeptide corresponding to an amino acid sequence within Dynamin which includes phosphorylated Ser774. Genename: DNM
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亚型
- IgG
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应用备注
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Immunofluorescence. Immunohistochemistry on Frozen Sections. Western Blot (1/1000): This antibody detects a band of approximately 95 kDa in Rathippocampus lysates. For the detection of Phosphoproteins, Serine Phosphatase inhibitors such as 10 mMSodium Fluoride should be added to the sample buffer. Milk or other casein-basedblocking solutions are not recommended as casein is a Phosphoprotein and its use canresult in high background.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- 10 mM HEPES, pH 7.5 containing 0.09 % Sodium Azide, 0.01 % BSA and 50 % Glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Dynamin 1 (DNM1)
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别名
- Dynamin-1
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背景
- Dynamin is a phosphoprotein expressed at nerve terminals, which plays an important role in the regulation of synaptic vesicle endocytosis. Cyclin dependent protein kinase 5 (cdk5) has been shown to phosphorylate serine 774 and 778 in vivo. Dephosphorylation of these residues is essential for endocytosis of synaptic vesicles.Synonyms: DNM, DNM1
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基因ID
- 140694
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NCBI登录号
- NP_542420
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UniProt
- P21575
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途径
- Toll-Like Receptors Cascades, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling
抗原
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