GAD 抗体 (C-Term)
(1 validation)Quick Overview for GAD 抗体 (C-Term) (ABIN3184725)
抗原
See all GAD (GAD1) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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特异性
- GAD67 Polyclonal Antibody detects endogenous levels of GAD67 protein.
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产品特性
- Rabbit Polyclonal to GAD67.
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纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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免疫原
- Synthesized peptide derived from the C-terminal region of human GAD67.
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亚型
- IgG
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anti-Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1) (AA 1-200) antibody
GAD1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1) (N-Term) antibodyGAD1 适用: 人 WB, IHC, ICC, IHC (p), IF, IHC (fro), IHC (ff), IHC (wm) 宿主: 兔 Polyclonal unconjugated
anti-Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1) (AA 1-97) antibodyGAD1 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1) (AA 455-594) antibodyGAD1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1) (Internal Region) antibodyVerified GAD1 适用: 人 WB, ELISA, IF, FACS 宿主: 山羊 Polyclonal unconjugated
anti-Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1) (AA 72-135) antibodyGAD1 适用: 人 WB, IHC, ELISA, Coat, StM 宿主: 小鼠 Monoclonal GAD1-2391 unconjugated
anti-Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1) (C-Term) antibodyGAD1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1) (AA 72-135) antibodyGAD1 适用: 人 WB, ELISA, FACS, Coat 宿主: 小鼠 Monoclonal GAD1-2563 unconjugated
anti-Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1) (AA 14-122) antibodyGAD1 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1) (C-Term) antibodyGAD1 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
- WB 1:500-1:2000, IHC 1:100-1:300, ELISA 1:10000,
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限制
- 仅限研究用
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- by
- Prof. Merighi, Laboratory of Neurobiology, Department of Veterinary Sciences, University of Turin
- No.
- #103754
- 日期
- 2019.12.12
- 抗原
- GAD1
- Lot Number
- Method validated
- Immunohistochemistry
- Positive Control
6 µm glass-mounted microtome sections from adult (24 months) mouse brain
- Negative Control
Slices incubated overnight with the blocking solution only and then processed with the secondary antibody
- Notes
Passed. The anti-GAD antibody ABIN3184725 specifically recognized GAD1 at a dilution of 1:200.
- Primary Antibody
- ABIN3184725
- Secondary Antibody
- goat anti-rabbit AF594-conjugated antibody (Thermo Fisher Scientific, A11029, lot 1942237)
- Full Protocol
- Perfuse mouse with 4% paraformaldehyde in 0.1 M phosphate buffer (PB) pH7.4 and post-fix spinal cord and brain samples in the same fixative for 2 h at RT.
- Wash samples in PBS, dehydrate, and embed samples in paraffin wax.
- Cut spinal cord and brain samples with a microtome in 6 µm-thick sections and mount sections on glass slides.
- After paraffin removal, block sections in PBS containing 1% albumin from chicken egg white (Sigma, A5378) and 0.3% Triton-X-100 (BioRad, 161-0407, lot 00583) 1 h at RT.
- Incubate sections with primary rabbit anti-GAD antibody (antibodies-online, ABIN3184725, lot 31965) diluted 1:200 in blocking solution ON at RT.
- Wash sections 3x 5 min in 0.01 M PBS.
- Incubate sections with secondary goat anti-rabbit AF594-conjugated antibody (Thermo Fisher Scientific, A11029, lot 1942237) diluted 1:200 in PBS for 1 h at RT.
- Wash sections 3x 5 min in 0.01 M PBS.
- Mount specimens in Fluoroshield (Sigma, F6182, lot MKCB0153V).
- Acquire images with Leica DM 6000B fluorescence microscope equipped with a digital camera at 40x magnification. Maintain Parameters for image acquisition (Exposure 2.2 s, Gain 2.3x, Saturation 1.5, Gamma 1.10) unchanged for all images.
- Experimental Notes
Dilutions of 1:50, 1:100, and 1:500 were also tested but 1:200 gave the best results.
生效 #103754 (Immunohistochemistry)!['独立验证'标志]( "成功验证")
!['独立验证'标志]( "成功验证")
Validation Images![A and B. Detection of GAD1+ neurons in the cortex using ABIN3184725.]()
A and B. Detection of GAD1+ neurons in the cortex using ABIN3184725.
Full Methods -
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freeze/thaw cycles.
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储存条件
- -20 °C
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储存方法
- Store at -20°C.
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- GAD (GAD1) (Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1))
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别名
- GAD67
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分子量
- 67 kDa
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基因ID
- 2571
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UniProt
- Q99259
抗原
-
