PDCD4 抗体 (C-Term)
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Quick Overview for PDCD4 抗体 (C-Term) (ABIN318336)
抗原
See all PDCD4 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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特异性
- The antibody reacts with Pdcd4 protein that is either phosphorylated or non-phosphorylated at Ser457.
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交叉反应 （详细）
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Species reactivity (expected):A BLAST analysis was used to suggest cross-reactivity with Pdcd4 from Human, Mouse, Rat and Xenopus based on 100 % homology with the immunizing sequence.
Species reactivity (tested):Human, Mouse, Rat, S. aureus, Xenopus. -
纯化方法
- Affinity Chromatography.
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免疫原
- Synthetic peptide corresponding amino acids near the carboxyl terminus of Human Pdcd4 protein.
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anti-Programmed Cell Death 4 (PDCD4) (C-Term) antibody
PDCD4 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Programmed Cell Death 4 (PDCD4) (pSer457) antibodyPDCD4 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Programmed Cell Death 4 (PDCD4) (AA 346-446) antibodyPDCD4 适用: 人 WB, IHC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Programmed Cell Death 4 (PDCD4) (AA 1-100) antibodyPDCD4 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Programmed Cell Death 4 (PDCD4) (AA 1-469) antibodyPDCD4 适用: 人 WB, ELISA, IHC, IHC (p) 宿主: 小鼠 Monoclonal K4C1 unconjugated
anti-Programmed Cell Death 4 (PDCD4) (AA 121-149) antibodyPDCD4 适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB33836 unconjugated
anti-Programmed Cell Death 4 (PDCD4) (AA 1-469) antibodyPDCD4 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Programmed Cell Death 4 (PDCD4) (pSer67) antibodyPDCD4 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Programmed Cell Death 4 (PDCD4) (N-Term) antibodyPDCD4 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Programmed Cell Death 4 (PDCD4) antibodyPDCD4 适用: 人 WB, IHC 宿主: 兔 Monoclonal unconjugated
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应用备注
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ELISA: 1/10,000-1/50,000. Western blot: 1/1,000-1/10,000 (a band approximately 52 kDa in size corresponding toPdcd4 protein in the appropriate cell lysate or extract). Immunohistochemistry: 1: 100-1/300.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.06 mg/mL (by UV absorbance at 280 nm)
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缓冲液
- 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, containing 0.01 % (w/v) Sodium Azide as preservative
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- PDCD4 (Programmed Cell Death 4 (PDCD4))
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别名
- PDCD4
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背景
- Programmed cell death 4 (Pdcd4) is a novel tumor supressor. Pdcd4 directly inhibits the helicase activity of eukaryotic translation initiation factor 4A (eIF4A), a component of the translation initiation complex. Pdcd4 also suppresses the transactivation of activator protein-1 (AP-1)-responsive promoters by c-Jun. Pdcd4 contains two Akt phosphorylation sites, one at Ser67 and the other at Ser457. The phosphorylation of Pdcd4 by Akt causes nuclear translocation of Pdcd4 and a significant decrease in the ability of Pdcd4 to interfere with the transactivation of AP-1-responsive promoters by c-Jun.Synonyms: H731, Neoplastic transformation inhibitor protein, Nuclear antigen H731-like, Programmed cell death protein 4
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基因ID
- 27250
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NCBI登录号
- NP_055271
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UniProt
- Q53EL6
抗原
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