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CDC25C 抗体 (pSer216)

This 兔 多克隆 antibody specifically detects CDC25C in WB 和 IHC (p). It exhibits reactivity toward 人.
产品编号 ABIN317983
发货至: 中国
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Quick Overview for CDC25C 抗体 (pSer216) (ABIN317983)

抗原

See all CDC25C 抗体
CDC25C (Cell Division Cycle 25 Homolog C (S. Pombe) (CDC25C))

适用

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宿主

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克隆类型

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多克隆

标记

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This CDC25C antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
  • 抗原表位

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    pSer216

    特异性

    P-CDC25C (pSer216) antibody detects endogenous levels of p-CDC25C protein.

    纯化方法

    Affinity chromatography
  • 应用备注

    ELISA: 1: 10000approx. 1: 20000. IHC: 1: 50approx. 1: 200.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 浓度

    1,0 mg/mL

    缓冲液

    Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2.

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C/-20 °C

    储存方法

    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • 抗原

    CDC25C (Cell Division Cycle 25 Homolog C (S. Pombe) (CDC25C))

    别名

    CDC25C

    背景

    Cell cycle events are regulated by the sequential activation and deactivation of cyclin-dependent kinases (Cdks), including Cdk2 and Cdc2. Cdk2, in complexes with cyclin E and cyclin A, appears necessary for the onset and progression of DNA replication, while the Cdc2 kinase, in complexes with cyclin A or cyclin B, is required for the initiation of cell division. Wee 1 has been identified as a protein kinase that suppresses the entry into mitosis by mediating inhibiting tyrosine phosphorylation of Cdc2 p34. In contrast, members of the Cdc25 family of protein phosphatases function as mitotic activators by dephosphorylation of Cdc2 p34 on regulatory tyrosine and possibly threonine residues. The Cdc25 gene family consists of at least three members that share approximately 40 % identity in their most conserved carboxy-terminal sequences.Synonyms: Cell Division Cycle 25C, Dual specificity phosphatase Cdc25C, M-phase inducer phosphatase 3

    分子量

    approx. 53 kDa

    基因ID

    995

    NCBI登录号

    NP_001781

    UniProt

    P30307

    途径

    Cell Division Cycle, M Phase
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