CDC25C 抗体 (pSer216)
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北京 101111
Quick Overview for CDC25C 抗体 (pSer216) (ABIN317983)
抗原
See all CDC25C 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pSer216
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特异性
- P-CDC25C (pSer216) antibody detects endogenous levels of p-CDC25C protein.
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纯化方法
- Affinity chromatography
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应用备注
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ELISA: 1: 10000approx. 1: 20000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1,0 mg/mL
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缓冲液
- Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- CDC25C (Cell Division Cycle 25 Homolog C (S. Pombe) (CDC25C))
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别名
- CDC25C
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背景
- Cell cycle events are regulated by the sequential activation and deactivation of cyclin-dependent kinases (Cdks), including Cdk2 and Cdc2. Cdk2, in complexes with cyclin E and cyclin A, appears necessary for the onset and progression of DNA replication, while the Cdc2 kinase, in complexes with cyclin A or cyclin B, is required for the initiation of cell division. Wee 1 has been identified as a protein kinase that suppresses the entry into mitosis by mediating inhibiting tyrosine phosphorylation of Cdc2 p34. In contrast, members of the Cdc25 family of protein phosphatases function as mitotic activators by dephosphorylation of Cdc2 p34 on regulatory tyrosine and possibly threonine residues. The Cdc25 gene family consists of at least three members that share approximately 40 % identity in their most conserved carboxy-terminal sequences.Synonyms: Cell Division Cycle 25C, Dual specificity phosphatase Cdc25C, M-phase inducer phosphatase 3
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分子量
- approx. 53 kDa
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基因ID
- 995
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NCBI登录号
- NP_001781
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UniProt
- P30307
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途径
- Cell Division Cycle, M Phase
抗原
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