MARCKS 抗体
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北京 101111
Quick Overview for MARCKS 抗体 (ABIN317779)
抗原
See all MARCKS 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- MARCKS antibody detects endogenous levels of MARCKS protein.(region surrounding Lys152)
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纯化方法
- Affinity chromatography
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应用备注
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ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1,0 mg/mL
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缓冲液
- Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- MARCKS (Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS))
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别名
- MARCKS
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背景
- Myristoylated alanine-rich protein kinase C substrate (MARCKS), also designated 80K or 80K-L, has been identified as a major cellular substrate for protein kinase C. Human MARCKS is a 332 amino acid protein with a calculated molecular weight of 31.534 kDa, however, it has been shown to run at 80-87 kDa on Western blot. The plasma membrane bound protein dissociates from the membrane upon phosphorylation by various PKC isoforms. In NIH/3T3 fibroblasts, PKC α and PKC ε, but not PKC δ, are responsible for MARCKS phosphorylation. MARCKS has been found to bind calmodulin, Actin and Synapsin and is a filamentous (F) Actin crosslinking protein.Synonyms: MACS, Myristoylated alanine-rich C-kinase substrate, PRKCSL
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分子量
- approx. 45 kDa
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基因ID
- 4082
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NCBI登录号
- NP_002347
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UniProt
- P29966
抗原
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