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LIPE 抗体

The 兔 多克隆 anti-LIPE antibody is suitable to detect LIPE in samples from 人, 小鼠 和 大鼠. It has been validated for WB, IHC (p) 和 EIA.
产品编号 ABIN317711
发货至: 中国
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中国
北京 101111
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Beijing Economic Technological Development Area
Room 801-803
4A Biotech Co.,Ltd.
Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for LIPE 抗体 (ABIN317711)

抗原

See all LIPE 抗体
LIPE (Lipase, Hormone-Sensitive (LIPE))

适用

  • 71
  • 58
  • 54
  • 2
  • 1
人, 小鼠, 大鼠

宿主

  • 86
  • 5
  • 3

克隆类型

  • 80
  • 14
多克隆

标记

  • 51
  • 6
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This LIPE antibody is un-conjugated

应用范围

  • 71
  • 39
  • 31
  • 15
  • 14
  • 14
  • 10
  • 7
  • 7
  • 4
  • 4
  • 2
  • 1
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
  • 特异性

    This antibody detects endogenous levels of HSL protein.(region surrounding Ala546)

    纯化方法

    Affinity Chromatography using epitope-specific immunogen.
  • 应用备注

    ELISA: 1/5000-1/10000. Western Blot: 1/500-1/1000. Immunohistochemistry: 1/50-1/200.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 浓度

    1.0 mg/mL

    缓冲液

    Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C/-20 °C

    储存方法

    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • 抗原

    LIPE (Lipase, Hormone-Sensitive (LIPE))

    别名

    LIPE

    背景

    HSL (hormone-sensitive lipase), a cytosolic neutral lipase regulated by reversible phosphorylation, catalyzes the rate limiting step in triglyceride lipolysis. HSL hydrolyzes stored triglycerides to free fatty acids in adipose and heart tissues. In organs with steroidogenic tissues, such as small intestine, HSL converts cholesteryl esters to free cholesterol for steroid hormone production. HSL is highly expressed in jejunal enterocytes and in the mucosa of the small intestine. Two major isoforms of HSL have been described resulting from the use of alternative translational start codons. The short isoform is expressed in adipose tissue while the long isoform is expressed in steroidogenic tissues such as testis. The long isoform, often referred to as testicular HSL contains an N-terminus of approximately 300 amino acids not present in the short isoform of HSL.Synonyms: EC=3.1.1.79, HSL, Hormone-sensitive lipase, LIPE

    分子量

    approx. 115.0 kDa

    基因ID

    3991

    NCBI登录号

    NP_005348

    UniProt

    Q05469

    途径

    AMPK Signaling, Monocarboxylic Acid Catabolic Process, Lipid Metabolism
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