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HSP70 抗体

This anti-HSP70 antibody is a 兔 多克隆 antibody detecting HSP70 in WB, IHC (p) 和 EIA. Suitable for 人, 小鼠 和 大鼠.
产品编号 ABIN317710
发货至: 中国

Quick Overview for HSP70 抗体 (ABIN317710)

抗原

See all HSP70 抗体
HSP70 (Heat Shock Protein 70 (HSP70))

适用

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人, 小鼠, 大鼠

宿主

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克隆类型

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多克隆

标记

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This HSP70 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
  • 特异性

    This antibody detects endogenous levels of HSP70 protein.(region surrounding Phe245)

    纯化方法

    Affinity Chromatography using epitope-specific immunogen.
  • 应用备注

    ELISA: 1/60000approx. 1/80000. Western Blot: 1/500approx. 1/1000. Immunohistochemistry: 1/50approx. 1/200.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 浓度

    1.0 mg/mL

    缓冲液

    Phosphate buffered saline (PBS), pH ~7.2 containing 0.05 % Sodium Azide as preservative.

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C/-20 °C

    储存方法

    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • 抗原

    HSP70 (Heat Shock Protein 70 (HSP70))

    别名

    Heat Shock Protein 70 / HSP70

    背景

    The HSP 70 family is composed of four highly conserved proteins: HSP 70, HSC 70, GRP 75 and GRP 78. These proteins serve a variety of roles: they act as molecular chaperones facilitating the assembly of multi-protein complexes, participate in the translocation of polypeptides across cell membranes and to the nucleus and aid in the proper folding of nascent polypeptide chains. All members of the family, except HSP 70, are constitutively expressed in primate cells. HSP 70 expression is strongly induced in response to heat stress. HSP 70 and HSC 70 play key roles in the cytosolic endoplasmic reticulum and mitochondrial import machinery and are found in both the cytosol and nucleus of mammalian cells. Both HSP 70 and HSC 70 are involved in the chaperoning of nascent polypeptide chains and in protecting cells against the accumulation of improperly folded proteins. GRP 78 is localized in the endoplasmic reticulum, where it receives imported secretory proteins and is involved in the folding and translocation of nascent peptide chains. It has been postulated that members of the HSP 70 family act as force-generating motors, relying on the hydrolysis of ATP for their activity.Synonyms: HSP70-1/HSP70-2, HSP70.1, HSPA1, HSPA1A, HSPA1B, Heat shock 70 kDa protein 1A/1B

    分子量

    approx. 68.0 kDa

    基因ID

    3303

    NCBI登录号

    NP_005336

    UniProt

    P08107
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