ELISA: 1: 5000approx. 1: 20000. WB: 1: 500approx. 1: 1000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
1,0 mg/mL
缓冲液
Phosphate buffered saline (PBS) with 0.05 % Sodium azide
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
The muscarinic acetylcholine receptors (mAChR) mediate a variety of cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels. The mAChRs transducer signals by coupling to G-proteins, which then modulate several downstream effector proteins and ion channels. Five mAChR subtypes have been identified, designated M1 to M5. The five receptor subtypes show distinct patterns of tissue distribution, as well as distinct pharmacological and functional properties. The amino acid sequence of each mAChR subtype reflects a structure that is characteristic of G-protein coupled receptors, consisting of seven highly conserved transmembrane segments and a large intracellular region unique to each subtype, which constitutes the effector-coupling domain.Synonyms: CHRM1, mAChR M1, mAChR-M1