BRF1 抗体
Quick Overview for BRF1 抗体 (ABIN317615)
抗原
See all BRF1 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody detects endogenous levels of TFIIIB90-1 protein.(region surrounding Glu257)
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纯化方法
- Affinity Chromatography using epitope-specific immunogen
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anti-BRF1, RNA polymerase III transcription initiation factor subunit (BRF1) (Internal Region) antibody
BRF1 适用: 人, 小鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-BRF1, RNA polymerase III transcription initiation factor subunit (BRF1) (AA 1-473) antibodyBRF1 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-BRF1, RNA polymerase III transcription initiation factor subunit (BRF1) antibodyBRF1 适用: 人 ICC, DB 宿主: 小鼠 Monoclonal BRF1G2A8 unconjugated
anti-BRF1, RNA polymerase III transcription initiation factor subunit (BRF1) (Center) antibodyBRF1 适用: 人, 小鼠, 大鼠 WB, IHC, IF, IC 宿主: 兔 Polyclonal unconjugated
anti-BRF1, RNA polymerase III transcription initiation factor subunit (BRF1) (AA 231-280) antibodyBRF1 适用: 人, 小鼠 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-BRF1, RNA polymerase III transcription initiation factor subunit (BRF1) (AA 501-600) antibodyBRF1 适用: 大鼠 WB, ELISA, IF (cc), IF (p), IHC (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-BRF1, RNA polymerase III transcription initiation factor subunit (BRF1) (Internal Region) antibodyBRF1 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-BRF1, RNA polymerase III transcription initiation factor subunit (BRF1) (AA 551-600) antibodyBRF1 适用: 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-BRF1, RNA polymerase III transcription initiation factor subunit (BRF1) (AA 1-473) antibodyBRF1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-BRF1, RNA polymerase III transcription initiation factor subunit (BRF1) (AA 1-208) antibodyBRF1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2E9 unconjugated
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应用备注
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Western Blot: 1/500-1/1000. ELISA: 1/1000-1/5000. Immunohistochemistry: 1/50-1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- BRF1 (BRF1, RNA polymerase III transcription initiation factor subunit (BRF1))
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别名
- BRF1 / TFIIIB90
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背景
- RNA polymerase (pol) III synthesizes tRNA, 5s rRNA, 7SL RNA and U6 snRNA and is overexpressed in many transformed cell lines and tumors in vivo, since cells must duplicate its protein components before division. Therefore, in order to maintain rapid growth, cells must produce a high level of Pol III transcribed RNA, which requires the presence of the TFIIIB and TFIIIC2 transcription factor complexes. The TFIIIC2 complex is composed of five subunits, TFIIIC220, TFIIIC110, TFIIIC102, TFIIIC90 and TFIIIC63, that are overexpressed in adenovirus transformed cells as well as in alignant cells in vivo, such as ovarian carcinomas. TFIIIC2 recruits RNA pol III and TFIIIB to promoter elements and may be a key component in the deregulation of malignant cells. The TFIIIB complex includes the TATA-binding protein (TBP), TFIIB-related factor 1 (TFIIIB90, BRF1) and TFIIIB, the expression of which are also upregulated in transformed cells. In many carcinomas, the tumor suppressors retinoblastoma (RB) and p53 are inactivated, which affects their ability to bind and inactivate the function of TFIIIB.Synonyms: B-related factor 1, BRF, GTF3B, RNA polymerase III subunit 2, TAF3B2, TAF3C, TATA box-binding protein-associated factor, Transcription factor IIIB 90 kDa subunit
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分子量
- approx. 74 kDa
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基因ID
- 2972
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NCBI登录号
- NP_001510
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UniProt
- Q92994
抗原
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