RYR1 抗体 (C-Term)
Quick Overview for RYR1 抗体 (C-Term) (ABIN317452)
抗原
See all RYR1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- C-Term
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特异性
- This antibody reacts with the C-terminus cytoplasmic domain of Ryanodine Receptor. Ryanodine receptors have been shown to play critical roles in the intracellular Ca2+ signaling occurring during cell activation in muscle cells and non-muscle cells. This antibody reacts with Ryanodine Receptor (~500 kDa) isolated from a variety of cell types (e.g. lymphocytes, macrophages, granulocytes, fibroblasts, epithelial, endothelial cells, skeletal muscle, cardiac muscle and brain tissues).
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纯化方法
- Purified
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免疫原
- Short synthetic polypeptide corresponding to the C-terminal domain of the ryanodinereceptor
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亚型
- IgG2b
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anti-Ryanodine Receptor 1 (Skeletal) (RYR1) (AA 1371-1386), (Intracellular) antibody
RYR1 适用: 人 WB, IHC, IF, IC 宿主: 兔 Polyclonal unconjugated
anti-Ryanodine Receptor 1 (Skeletal) (RYR1) antibodyRYR1 适用: 人, 小鼠, 大鼠 ELISA, WB 宿主: 兔 Polyclonal unconjugated
anti-Ryanodine Receptor 1 (Skeletal) (RYR1) (AA 1-534) antibodyRYR1 适用: 人 ELISA, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunohistochemistry on frozen sections: 1: 100 (Use indirect immunofluorescence. Visualization by confocal microscopy is required, as detection by standard fluorescentmicroscopy will not be adequate to detect the RyR. Additionally, fluorescent, notenzymatic, detection is required. Due to the intensity of confocal lasers, use of ananti-fading agent, such as DABCO, is strongly recommended. ). Immunofluorescence: 1: 100. ELISA: 1: 1000. Flow Cytometry: 1: 500. Western blot: 1: 100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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缓冲液
- PBS, 0.09 % Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C/-20 °C
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储存方法
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Store the antibody undiluted at 2 - 8 °C up to one month or (in aliquots) at -20 °C forlonger. Avoid repeated freezing and thawing. Should this product contain a precipitate werecommend microcentrifugation before use.
Shelf life: one year from despatch. -
有效期
- 12 months
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- RYR1 (Ryanodine Receptor 1 (Skeletal) (RYR1))
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别名
- Ryanodine Receptor 1 / RYR1
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基因ID
- 6261
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UniProt
- P21817
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途径
- Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development
抗原
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