RSV 抗体
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北京 101111
Quick Overview for RSV 抗体 (ABIN317238)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
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产品特性
- Synonyms: RSV, HRSV, Fusion protein, Fusion glycoprotein F0, Protein F
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纯化方法
- Purified IgG prepared by affinity chromatography on protein A from tissue culture supernatant
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亚型
- IgG2b
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anti-Respiratory Syncytial Virus (RSV) (AA 151-250) antibody
RSV 适用: 人, Human respiratory syncytial virus A, Virus IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Respiratory Syncytial Virus (RSV) antibodyRSV 适用: Respiratory Syncytial Virus (RSV) ELISA, IF, WB, Neut, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
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ELISA: 1/20-1/200. Immunofluorescence: 1/10-1/50.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 0.1 mg/mL
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缓冲液
- Phosphate buffered saline pH 7.2 containing 0.09 % Sodium Azide as preservative.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C/-20 °C
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储存方法
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Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. -
有效期
- 12 months
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- RSV (Respiratory Syncytial Virus (RSV))
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别名
- Respiratory Syncytial Virus / RSV
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物质类
- Virus
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背景
- Respiratory Syncytial Virus (RSV) Fusion (F) Glycoprotein is a Class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the heptad repeat (HR) regions assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes. Directs fusion of viral and cellular membranes leading to delivery of the nucleocapsid into the cytoplasm. This fusion is pH independent and occurs directly at the outer cell membrane. The trimer of F1-F2 (protein F) interacts with glycoprotein G at the virion surface. Upon binding of G to heparan sulfate, the hydrophobic fusion peptide is unmasked and interacts with the cellular membrane, inducing the fusion between host cell and virion membranes. Notably, RSV fusion protein is able to interact directly with heparan sulfate and therefore actively participates in virus attachment. Furthermore, the F2 subunit was identifed as the major determinant of RSV host cell specificity. Later in infection, proteins F expressed at the plasma membrane of infected cells mediate fusion with adjacent cells to form syncytia, a cytopathic effect that could lead to tissue necrosis. The fusion protein is also able to trigger p53-dependent apoptosis.
抗原
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