VAMP2 抗体 (N-Term)
Quick Overview for VAMP2 抗体 (N-Term) (ABIN317061)
抗原
See all VAMP2 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 1-89, N-Term
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特异性
- This antibody recognizes specifically Synaptobrevin 2, but it also shows a low affinity to Synaptobrevin 1. Detects a band of approximately 14 kDa in rat brain lysates.
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产品特性
- Synonyms: VAMP2, Vesicle-associated membrane protein 2, SYB2
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纯化方法
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
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免疫原
- Recombinant Human Synaptobrevin 2 (1-89 aa) purified from E. coli.
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亚型
- IgG1
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anti-Vesicle-Associated Membrane Protein 2 (Synaptobrevin 2) (VAMP2) (AA 1-100) antibody
VAMP2 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Vesicle-Associated Membrane Protein 2 (Synaptobrevin 2) (VAMP2) (AA 1-89) antibodyVAMP2 适用: 人 WB, IHC, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 3E5 unconjugated
anti-Vesicle-Associated Membrane Protein 2 (Synaptobrevin 2) (VAMP2) (AA 2-17) antibodyVAMP2 适用: 人, 小鼠, 大鼠, Hamster WB, IHC, ICC, IP 宿主: 豚鼠 Polyclonal unconjugated
anti-Vesicle-Associated Membrane Protein 2 (Synaptobrevin 2) (VAMP2) antibodyVAMP2 适用: 人, 小鼠, 大鼠 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Vesicle-Associated Membrane Protein 2 (Synaptobrevin 2) (VAMP2) (AA 1-89) antibodyVAMP2 适用: 人, 小鼠 WB, ELISA, ICC, IF 宿主: 小鼠 Monoclonal 3E5 unconjugated
anti-Vesicle-Associated Membrane Protein 2 (Synaptobrevin 2) (VAMP2) (N-Term) antibodyVAMP2 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Vesicle-Associated Membrane Protein 2 (Synaptobrevin 2) (VAMP2) (AA 1-94) antibodyVAMP2 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Vesicle-Associated Membrane Protein 2 (Synaptobrevin 2) (VAMP2) (AA 1-66) antibodyVAMP2 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Vesicle-Associated Membrane Protein 2 (Synaptobrevin 2) (VAMP2) (AA 1-89) antibodyVAMP2 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3E5 unconjugated
anti-Vesicle-Associated Membrane Protein 2 (Synaptobrevin 2) (VAMP2) (AA 1-50) antibodyVAMP2 适用: 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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ELISA. Western blot (1/2,000-1/3,000). Immunohistochemistry on Paraffin Sections (10 μg/mL). This antibody was validated foruse in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE)human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubationwith the primary antibody, slides were incubated with biotinylated secondary antibody,followed by alkaline phosphatase-streptavidin and chromogen.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
- PBS, pH 7.4, containing 0.09 % Sodium Azide as preservative.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C/-20 °C
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储存方法
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Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. -
有效期
- 12 months
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- VAMP2 (Vesicle-Associated Membrane Protein 2 (Synaptobrevin 2) (VAMP2))
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别名
- VAMP-2 / Synaptobrevin-2
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背景
- Synaptobrevin 2 (Syb2) also known as vesicle associated membrane protein-2 (VAMP2) is a membrane protein that belongs to the v-SNARE family of proteins, and is an essential component in neurotransmitter exocytosis from the presynaptic membrane. It is expressed on the synaptic vesicle, where it is bound by synaptophysin. Synaptophysin targets the vesicle to the presynaptic membrane and releases Syb2, which forms a stable complex with syntaxin and SNAP-25 (both expressed on the target membrane). Nerve cell depolarization and influx of calcium leads to vesicle fusion and neurotransmitter release into the synapse.Synonyms: SYB2, VAMP2, Vesicle-associated membrane protein 2
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基因ID
- 6844
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UniProt
- P63027
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途径
- Peptide Hormone Metabolism, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport
抗原
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