Transferrin Receptor 抗体
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- 抗原 See all Transferrin Receptor (TFRC) 抗体
- Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))
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适用
- 小鼠
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宿主
- 大鼠
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克隆类型
- 单克隆
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标记
- This Transferrin Receptor antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func)
- 特异性
- This antibody recognises CD71, a 95 kDa cell surface protein that is otherwise known as transferrin receptor. This antibody recognises native, soluble and denatured forms of murine CD71. Binding of the 8D3 antibody to CD71 does not inhibit the proliferation of cell lines tested, and does not interfere with the uptake of iron into cells. The antibody has been used as a BBB transporter vector in mice and is suitable for studying CD71 expression, and iron uptake into different tissues, in the Mouse.
- 产品特性
- Synonyms: TfR1, p90, Transferrin receptor protein 1
- 纯化方法
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- 内毒素水平
- < 0.01 Eu/μg
- 免疫原
- Mouse transformed endothelioma cell line t. end1. Spleen cells from immunised Lewis rats were fused with the cells of the mouse NSOmyeloma cell line
- 克隆位点
- 8D3
- 亚型
- IgG2a
- Top Product
- Discover our top product TFRC Primary Antibody
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- 应用备注
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Functional Assays. Flow Cytometry: Use 10 μL of 1/100-1/200 diluetd CD71 antibody to label 10^6 cells. Immunohistochemistry on Frozen Sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 说明
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Endotoxin Level: <0.01 Eu/μg
- 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- Phosphate buffered saline without preservatives
- 储存液
- Without preservative
- 储存条件
- -20 °C
- 储存方法
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Store the antibody undiluted at -20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. - 有效期
- 12 months
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- 抗原
- Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))
- 别名
- CD71 / TFRC (TFRC 产品)
- 背景
- CD71 is a major iron-binding protein, which plays a key role in the transport of iron into cells that require it. In mice, CD71 is widely expressed on a variety of cells, including Sertoli cells and cells which form the blood brain barrier (BBB) in the central nervous system.Synonyms: TfR1, Transferrin receptor protein 1, p90
- 基因ID
- 22042
- UniProt
- Q62351
- 途径
- Transition Metal Ion Homeostasis
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