STN8 Kinase (AA 425-438) 抗体
(1 reference)Our Local Distributor
北京 101111
Quick Overview for STN8 Kinase (AA 425-438) 抗体 (ABIN3155736)
抗原
适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- AA 425-438
-
原理
- Anti-STN8 | Serine/threonine-protein kinase STN8 (chloroplastic)
-
交叉反应 （详细）
-
Arabidopsis thaliana, Triticum aestivum
Not reactive in: No confirmed exceptions from predicted reactivity are currently known -
预测反应
- Oryza sativa
-
纯化方法
- Immunogen affinity purified serum
-
免疫原
- KLH-conugated synthetic peptide (amino acids 425-438) specific for Arabidopsis thaliana STN8 serine/threonine proteinkinase
-
-
anti-STN8 Kinase antibody
适用: Arabidopsis WB 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
- 1 : 2000 (WB)
-
说明
-
For best results with this antibody sample buffer needs to contain 6 M urea (138mM TrisHCl pH 6.8, 6M urea, 22.2% Glycerol, 4.3% SDS).STN8 is a nuclear encoded protein which is localized in chloroplast, hence posses a chloroplastic target peptides (cTP) at the beginning of the amino acid sequence which is cut off. Accroding to TargetP (program that predicts the length of the cTP:s) the length of cTP for Stn8 is 49 amino acids. STN8 portein mobility can be also affected by urea present in the gel.
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- For reconstitution add 50 μL of sterile water
-
缓冲液
- Immunogen affinity purified serum in PBS pH 7.4.
-
注意事项
- Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube
-
储存条件
- -20 °C
-
储存方法
- Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
-
-
-
: "Relationship between thyroid stimulating hormone and thyroid stimulating immunoglobulin in Graves' hyperthyroidism." in: Journal of endocrinological investigation, Vol. 34, Issue 3, pp. 222-4, (2011) (PubMed).
-
-
- STN8 Kinase
-
背景
-
Background: Serine/threonine protein kinase (EC=2.7.11.1), known as STN8 in Arabidopsis, and its Stl1 homologue in Chlamydomonas, are STN7/Stt7-like proteins that are not required for state transitions. The Arabidopsis mutants deficient in stn8 gene demonstrated highly reduced levels of the phosphorylated proteins of the photosystem II (Bonardi et al., 2005 Varionen et al., 2005). Alternative name: Protein STATE TRANSITION 8.
Additional information: An extract from STN8 mutant needs to be used in pararel to determine specific band of STN8 protein on a western blot
-
分子量
- 54.9 kDa or 46 kDa on 6 M urea gel
-
基因ID
- 831741
-
UniProt
- Q9LZV4
抗原
-
