VDAC1 抗体
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- 抗原 See all VDAC1 抗体
- VDAC1 (Voltage-Dependent Anion Channel 1 (VDAC1))
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适用
- Arabidopsis thaliana, Moss, Papaver, Spinach
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This VDAC1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunolocalization (IL), Blue-native PAGE (BN PAGE)
- 交叉反应 (详细)
- No cross-reactivity with: Chlamydomonas reinhardtii, Glycine max, Zea mays, diatoms
- 产品特性
- Expected / apparent Molecular Weight of the Antigene: 29 kDa (for Arabidopsis thaliana)
- 纯化方法
- serum
- 免疫原
- KLH-conjugated peptide conserved in all known higher plant VDAC1 proteins including Arabidopsis thaliana with the locus name: At3g01280
- Top Product
- Discover our top product VDAC1 Primary Antibody
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- 应用备注
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Recommended Dilution: 1 : 5000 on 2-30 µg of protein/lane with standard ECL (WB), 1 : 500 (IL).
Cellular [compartment marker] of mitochondrial outer membrane - 说明
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Amount of mitochondrial fraction detected by anti-VDAC1 antibody was from 2-10 µg.Immunolocalization method description and images are available hereBlue-native (2D BN/SDS-PAGE) methodology is described in Piechota et al. 2010
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- For reconstitution add 200 µL of sterile water.
- 缓冲液
- PBS pH 7.4
- 注意事项
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Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles. - 储存条件
- -20 °C
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Biogenesis of mitochondria in cauliflower (Brassica oleracea var. botrytis) curds subjected to temperature stress and recovery involves regulation of the complexome, respiratory chain activity, ..." in: Biochimica et biophysica acta, Vol. 1847, Issue 4-5, pp. 399-417, (2015) (PubMed).
: "Overexpression of UCP1 in tobacco induces mitochondrial biogenesis and amplifies a broad stress response." in: BMC plant biology, Vol. 14, pp. 144, (2014) (PubMed).
: "Ubiquitin-specific protease16 modulates salt tolerance in Arabidopsis by regulating Na(+)/H(+) antiport activity and serine hydroxymethyltransferase stability." in: The Plant cell, Vol. 24, Issue 12, pp. 5106-22, (2013) (PubMed).
: "CPD photolyase repairs ultraviolet-B-induced CPDs in rice chloroplast and mitochondrial DNA." in: The Plant journal : for cell and molecular biology, (2011) (PubMed).
: "Analysis of the Arabidopsis Cytosolic Proteome Highlights Subcellular Partitioning of Central Plant Metabolism." in: Journal of proteome research, (2010) (PubMed).
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Biogenesis of mitochondria in cauliflower (Brassica oleracea var. botrytis) curds subjected to temperature stress and recovery involves regulation of the complexome, respiratory chain activity, ..." in: Biochimica et biophysica acta, Vol. 1847, Issue 4-5, pp. 399-417, (2015) (PubMed).
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- 抗原
- VDAC1 (Voltage-Dependent Anion Channel 1 (VDAC1))
- 别名
- VDAC1 (VDAC1 产品)
- 背景
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AGI Code: At3g01280
VDAC1 protein (called also Synonymes: At3g01280, outer mitochondrial membrane protein porin 1, T22N4_9, T22N4.9, VDAC 1, Voltage-dependent anion-selective channel protein 1, voltage-gated ion-selective channel) forms a channel through the cell membrane for diffusion of small hydrophilic molecules. Evolutionary origin of VDAC protein is not clear and their structure and properties are quite different making those proteins only conceptually like porins (Clausen et al. 2004). - 分子量
- 29 kDa (for Arabidopsis thaliana)
- UniProt
- Q9SRH5
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