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VDAC1 抗体

Cited in 5+ publications. The 兔 多克隆 anti-VDAC1 antibody (ABIN3155731) specifically detects VDAC1 in WB 和 BN PAGE. The antibody is reactive with Arabidopsis thaliana, Oryza sativa, Papaver, Physcomytrium Patens, Spinach, Zea mays, Orange, Fortunella margarita, Cauliflower (Brassica oleracea var. botrytis), Brassica rapa, Beet 和 Amaranthus palmeri samples.
产品编号 ABIN3155731
发货至: 中国
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Quick Overview for VDAC1 抗体 (ABIN3155731)

抗原

See all VDAC1 抗体
VDAC1 (Voltage-Dependent Anion Channel 1 (VDAC1))

适用

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Arabidopsis thaliana, Oryza sativa, Papaver, Physcomytrium Patens, Spinach, Zea mays, Orange, Fortunella margarita , Cauliflower (Brassica oleracea var. botrytis), Brassica rapa, Beet, Amaranthus palmeri

宿主

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克隆类型

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多克隆

标记

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This VDAC1 antibody is un-conjugated

应用范围

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Western Blotting (WB), Blue-native PAGE (BN PAGE)
  • 原理

    Anti-VDAC1-5 | Voltage-dependent anion-selective channel protein 1-5

    交叉反应 (详细)

    Arabidopsis thaliana, Amaranthus palmeri, Beta vulgaris, Brassica oleracea var. botrytis, Brassica rapa subsp. rapa, Citrus sinensis, Fortunella margarita Swingle, Oryza sativa, Papaver sp. pollen tubes (IL), Spinacia oleracea, Physcomitrium patens, Zea mays
    Not reactive in: Chlamydomonas reinhardtii, Glycine max, diatoms, Saccharomyces cerevisiae

    预测反应

    Arabidopsis alpina,Aundo donax,Brachypodium distachyon,Brassica campestris,Brassica napus,Brassica rapa subsp. pekinensis,Capsella rubella,Citrus clementina,Eutrema salsugineum,Glycine max,Glycine soja,Gossypium arboreum,Hoedum vulgare var. distichum,Jatropha curcas,Medicago truncatula,Mesembryanthemum crystallinum,Morus notabilis,Nicotiana tabacum,Phaseolus coccineus,Phaseolus vulgaris,Pisum sativum,Plantago major,Prunus persica,Ricinus communis,Solanum lycopersicum,Solanum tuberosum,Sorghum bicolor,Theobroma cacao,Triticum aestivum,Vitis vinifera

    纯化方法

    Immunogen affinity purified serum

    免疫原

    KLH-conjugated peptide conserved in all known higher plant VDAC proteins including Arabidopsis thaliana VDAC1 UniProt: Q9SRH5
  • 应用备注

    1 : 500 (IL), 1 : 5000, 2-30 μg protein/lane (WB)

    说明

    Amount of mitochondrial fraction detected by anti-VDAC1 antibody was from 2-10 µg.Immunolocalization method description and images are available hereBlue-native (2D BN/SDS-PAGE) methodology is described in Piechota et al. 2010

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    For reconstitution add 50 μL of sterile water

    缓冲液

    Immunogen affinity purified serum in PBS pH 7.4.

    注意事项

    Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

    储存条件

    -20 °C

    储存方法

    Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
  • Rurek, Woyda-Ploszczyca, Jarmuszkiewicz et al.: "Biogenesis of mitochondria in cauliflower (Brassica oleracea var. botrytis) curds subjected to temperature stress and recovery involves regulation of the complexome, respiratory chain activity, ..." in: Biochimica et biophysica acta, Vol. 1847, Issue 4-5, pp. 399-417, (2015) (PubMed).

    Barreto, Okura, Neshich, Maia, Arruda: "Overexpression of UCP1 in tobacco induces mitochondrial biogenesis and amplifies a broad stress response." in: BMC plant biology, Vol. 14, pp. 144, (2014) (PubMed).

    Zhou, Zhao, Yang, Chen, Liu, Jin, Chen, Li, Deng, Schumaker, Guo: "Ubiquitin-specific protease16 modulates salt tolerance in Arabidopsis by regulating Na(+)/H(+) antiport activity and serine hydroxymethyltransferase stability." in: The Plant cell, Vol. 24, Issue 12, pp. 5106-22, (2013) (PubMed).

    Takahashi, Teranishi, Ishida, Kawasaki, Takeuchi, Yamaya, Watanabe, Makino, Hidema: "CPD photolyase repairs ultraviolet-B-induced CPDs in rice chloroplast and mitochondrial DNA." in: The Plant journal : for cell and molecular biology, (2011) (PubMed).

    Ito, Batth, Petzold, Redding-Johanson, Mukhopadhyay, Verboom, Meyer, Millar, Heazlewood: "Analysis of the Arabidopsis Cytosolic Proteome Highlights Subcellular Partitioning of Central Plant Metabolism." in: Journal of proteome research, (2010) (PubMed).

  • 抗原

    VDAC1 (Voltage-Dependent Anion Channel 1 (VDAC1))

    别名

    VDAC1

    背景

    Background: VDAC proteins are porin-type, beta-barrel diffusion pores. Prominently localized in the outer mitochondrial membrane and involved in metabolite exchange.

    Additional information: Cellular [compartment marker] of mitochondrial outer membrane for western blot,

    分子量

    29 kDa (for Arabidopsis thaliana)

    基因ID

    820914

    UniProt

    Q9M2W6-1, Q9SMX3-1, F4K3R8-1
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