5-Methylcytidine 抗体
FACS, IF, IHC (fro), IHC (p), ICC
宿主: 小鼠
Monoclonal
33D3
unconjugated
1 image
(2 references)-
- 抗原 See all 5-Methylcytidine products
- 5-Methylcytidine
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This 5-Methylcytidine antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)
- 特异性
- Modified base 5-methylcytidine
- 纯化方法
- Purified IgG from culture supernatant by Affinity chromatography on Protein A ceramic hyper D F - Filtration on 0.22 µm.
- 克隆位点
- 33D3
- 亚型
- IgG1
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anti-5-Methylcytidine antibody
DB 宿主: 兔 Monoclonal unconjugated
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- 应用备注
- MeDIP: Used 5ul in 200ul reaction with 2ug digested genomic DNA from Drosophila. Flow Cyt: Use 10ul of working dilution to label 1000000 cells in 100ul. ICC/IF: Used at a dilution of 1/200 for 16 hrs. IHC-P: Use at an assay dependent dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Sodium citrate buffer pH6.0 is recommended for this purpose. IHC-Fr: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
- 说明
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For research use only
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/ml
- 缓冲液
- Phosphate Buffered Saline 10 mM – NaCl 0.15 M – pH 7.4
- 储存条件
- -20 °C
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: "Functional analysis of male mouse haploid germ cells of various differentiation stages: early and late round spermatids are functionally equivalent in producing progeny." in: Biology of reproduction, Vol. 80, Issue 3, pp. 511-7, (2009) (PubMed).
: "Evidence for transgenerational transmission of epigenetic tumor susceptibility in Drosophila." in: PLoS genetics, Vol. 3, Issue 9, pp. 1598-606, (2007) (PubMed).
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: "Functional analysis of male mouse haploid germ cells of various differentiation stages: early and late round spermatids are functionally equivalent in producing progeny." in: Biology of reproduction, Vol. 80, Issue 3, pp. 511-7, (2009) (PubMed).
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- 抗原
- 5-Methylcytidine
- Abstract
- 5-Methylcytidine 产品
- 物质类
- Chemical
- 背景
- DNA methylation is a DNA modification process, which is involved in the control of gene expression. The 5-Methylcytidine antibody specifically binds the modified base 5-methylcytidine (5-MeCyd) found in DNA of plants and vertebrates.
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