5-Methylcytidine 抗体
This anti-5-Methylcytidine antibody is a 小鼠 单克隆 antibody detecting 5-Methylcytidine in FACS, IF, IHC (fro), IHC (p) 和 ICC. Suitable for . This Primary Antibody has been cited in 2+ publications.
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(2 references)Quick Overview for 5-Methylcytidine 抗体 (ABIN308218)
抗原
5-Methylcytidine
宿主
小鼠
克隆类型
单克隆
标记
This 5-Methylcytidine antibody is un-conjugated
应用范围
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)
克隆位点
33D3
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特异性
- Modified base 5-methylcytidine
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纯化方法
- Purified IgG from culture supernatant by Affinity chromatography on Protein A ceramic hyper D F - Filtration on 0.22 µm.
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亚型
- IgG1
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anti-5-Methylcytidine antibody
DB 宿主: 兔 Monoclonal unconjugated
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应用备注
- MeDIP: Used 5ul in 200ul reaction with 2ug digested genomic DNA from Drosophila. Flow Cyt: Use 10ul of working dilution to label 1000000 cells in 100ul. ICC/IF: Used at a dilution of 1/200 for 16 hrs. IHC-P: Use at an assay dependent dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Sodium citrate buffer pH6.0 is recommended for this purpose. IHC-Fr: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
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说明
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For research use only
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/ml
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缓冲液
- Phosphate Buffered Saline 10 mM – NaCl 0.15 M – pH 7.4
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储存条件
- -20 °C
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: "Functional analysis of male mouse haploid germ cells of various differentiation stages: early and late round spermatids are functionally equivalent in producing progeny." in: Biology of reproduction, Vol. 80, Issue 3, pp. 511-7, (2009) (PubMed).
: "Evidence for transgenerational transmission of epigenetic tumor susceptibility in Drosophila." in: PLoS genetics, Vol. 3, Issue 9, pp. 1598-606, (2007) (PubMed).
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: "Functional analysis of male mouse haploid germ cells of various differentiation stages: early and late round spermatids are functionally equivalent in producing progeny." in: Biology of reproduction, Vol. 80, Issue 3, pp. 511-7, (2009) (PubMed).
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- 5-Methylcytidine
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物质类
- Chemical
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背景
- DNA methylation is a DNA modification process, which is involved in the control of gene expression. The 5-Methylcytidine antibody specifically binds the modified base 5-methylcytidine (5-MeCyd) found in DNA of plants and vertebrates.
抗原
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