PAR1 抗体 (AA 27-102)
Quick Overview for PAR1 抗体 (AA 27-102) (ABIN307496)
抗原
See all PAR1 (F2R) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 27-102
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特异性
- Selected for its ability to recognize human PAR1 in the applications listed.
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纯化方法
- Affinity purified
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免疫原
- E. coli-derived recombinant human PAR1 (aa27-102, 375-428).
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亚型
- IgG
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应用备注
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Approved: ELISA, Flo, WB
Usage: Western Blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect human PAR1. The detection limit for rhPAR1 is approximately 5 ng/lane under non-reducing and reducing conditions. Flow Cytometry: This antibody was tested in flow cytometry using whole blood granulocytes. Dilute this antibody to 50 μg/mL and add 10 μL of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200 L. The binding of unlabeled antibodies may be visualized by adding a secondary developing reagent such as anti-goat IgG conjugated to a fluorochrome. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect human PAR1. The detection limit for rhPAR1 is approximately 0.2 ng/well. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- deionized water
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浓度
- Lot specific
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缓冲液
- Lyophilized.
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- PAR1 (F2R) (Coagulation Factor II (thrombin) Receptor (F2R))
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别名
- F2R / Thrombin Receptor / PAR1
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背景
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Name/Gene ID: F2R
Subfamily: Proteinase-activated
Family: GPCR
Synonyms: F2R, CF2R, Coagulation factor II receptor, PAR1, PAR-1, Thrombin receptor, TR, HTR, Protease-activated receptor 1 -
基因ID
- 2149
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UniProt
- P25116
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途径
- Nuclear Receptor Transcription Pathway, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Protein targeting to Nucleus
抗原
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