ELAVL1 抗体 (AA 1-13)
Quick Overview for ELAVL1 抗体 (AA 1-13) (ABIN307186)
抗原
See all ELAVL1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1-13
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品牌
- IHC-plus™
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特异性
- Recognizes human HuR, Mr ~36kD.
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预测反应
- Percent identity by BLAST analysis: Human, Gorilla, Monkey, Mouse, Rat, Sheep, Elephant, Panda, Dog, Bat, Bovine, Rabbit, Horse, Pig, Opossum, Chicken (100%) Gibbon, Platypus (92%).
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纯化方法
- Affinity purified
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免疫原
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KLH-conjugated, synthetic peptide corresponding to aa1-13 (MSNGYEDHMAEDC) of human HuR. The immunizing sequence is identical in chicken, mouse, and rat. Percent identity by BLAST analysis: Human, Gorilla, Monkey, Mouse, Rat, Sheep, Elephant, Panda, Dog, Bat, Bovine, Rabbit, Horse, Pig, Opossum, Chicken (100%), Gibbon, Platypus (92%).
Type of Immunogen: Synthetic peptide - KLH conjugated -
亚型
- IgG
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应用备注
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Approved: IHC, IHC-P (5 μg/mL), IP, WB
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: 0.5-2.0 μg/mL. Detected HuR in RIPA lysates from 3T3/A31 cells. Immunoprecipitation: 4 μg immunoprecipitated HuR from 500 μg of 3T3/A31 RIPA lysate. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- Supplied as a liquid in Tris-glycine, 0.15M sodium chloride, 0.05 % sodium azide, pH 7.4, 30 % glycerol
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeat freeze-thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- ELAVL1 (ELAV (Embryonic Lethal, Abnormal Vision, Drosophila)-Like 1 (Hu Antigen R) (ELAVL1))
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别名
- ELAVL1 / HUR
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背景
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Name/Gene ID: ELAVL1
Synonyms: ELAVL1, ELAV1, Hua, HUR, Hu antigen R, ELAV-like protein 1, Hu-antigen R, MelG -
基因ID
- 1994
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UniProt
- Q15717
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途径
- AMPK Signaling
抗原
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