GAL4 Activation Domain 抗体
适用: Saccharomyces cerevisiae
WB, IP
宿主: 小鼠
Monoclonal
5C8-5
unconjugated
-
- 抗原
- GAL4 Activation Domain
- 适用
- Saccharomyces cerevisiae
-
宿主
- 小鼠
-
克隆类型
- 单克隆
-
标记
- 非结合性
- 应用范围
- Western Blotting (WB), Immunoprecipitation (IP)
- 交叉反应
- Saccharomyces cerevisiae
- 产品特性
- GAL4 is a transcriptional activator which activates genes necessary for galactose catabolism. The DNA binding domain of GAL4 is contained in the first 74 amino acids and contains a zinc finger sequence motif. However, amino acids 1-147 have been shown to bind to DNA in vitro. While GAL4 (1-74) bind DNA specifically, it is with a lower affinity than the GAL4 (1-147). GAL4 (1-147) binds to a specific 17 base pair nucleotide sequence, but alone is insufficient to activate transcription. Either of the two acidic activating regions, activating region I (amino acids 148-238) or activating region II (amino acids 768-881), must be joined to activate transcription.
- 纯化方法
- Protein A/G Chromatography
- 免疫原
- Hybridoma produced by the fusion of splenocytes from mice immunized with full length GAL4 expressed in E. Coli and mouse myeloma cells.
- 克隆位点
- 5C8-5
- 亚型
- IgG1
-
anti-GAL4 Activation Domain (Activation Site) antibody
适用: Saccharomyces cerevisiae WB 宿主: 兔 Polyclonal unconjugated
-
- 应用备注
- Antibody can be used for Western Blotting (5-10 μg/mL). Recognizes GAL4 derivatives containing the C-terminal activation domain. Optimal concentration should be evaluated by serial dilutions.
- 限制
- 仅限研究用
-
- 缓冲液
- Provided as solution in phosphate buffered saline with 0.08 % sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
-
- 抗原
- GAL4 Activation Domain
- 别名
- GAL-4 Activation Domain
-
You are here:
