GAL4 Activation Domain 抗体
This 小鼠 单克隆 antibody specifically detects GAL4 Activation Domain in WB 和 IP. It exhibits reactivity toward Saccharomyces cerevisiae.
产品编号 ABIN307168
发货至:
中国
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Quick Overview for GAL4 Activation Domain 抗体 (ABIN307168)
抗原
GAL4 Activation Domain
适用
Saccharomyces cerevisiae
宿主
小鼠
克隆类型
单克隆
标记
非结合性
应用范围
Western Blotting (WB), Immunoprecipitation (IP)
克隆位点
5C8-5
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交叉反应
- Saccharomyces cerevisiae
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产品特性
- GAL4 is a transcriptional activator which activates genes necessary for galactose catabolism. The DNA binding domain of GAL4 is contained in the first 74 amino acids and contains a zinc finger sequence motif. However, amino acids 1-147 have been shown to bind to DNA in vitro. While GAL4 (1-74) bind DNA specifically, it is with a lower affinity than the GAL4 (1-147). GAL4 (1-147) binds to a specific 17 base pair nucleotide sequence, but alone is insufficient to activate transcription. Either of the two acidic activating regions, activating region I (amino acids 148-238) or activating region II (amino acids 768-881), must be joined to activate transcription.
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纯化方法
- Protein A/G Chromatography
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免疫原
- Hybridoma produced by the fusion of splenocytes from mice immunized with full length GAL4 expressed in E. Coli and mouse myeloma cells.
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亚型
- IgG1
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anti-GAL4 Activation Domain (Activation Site) antibody
适用: Saccharomyces cerevisiae WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- Antibody can be used for Western Blotting (5-10 μg/mL). Recognizes GAL4 derivatives containing the C-terminal activation domain. Optimal concentration should be evaluated by serial dilutions.
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限制
- 仅限研究用
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缓冲液
- Provided as solution in phosphate buffered saline with 0.08 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
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- GAL4 Activation Domain
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别名
- GAL-4 Activation Domain
抗原
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