SRPR 抗体 (AA 39-295)
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北京 101111
Quick Overview for SRPR 抗体 (AA 39-295) (ABIN307162)
抗原
See all SRPR 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 39-295
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交叉反应
- 犬
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产品特性
- Signal Recognition Particle Receptor, α subunit,The receptor for Signal Recognition Particle (SRP) is the site on the endoplasmic reticulum that ribosomes translating secreted and integral membrane proteins are initially targeted to. Once the ribosome-SRP complex arrives at the SRP receptor the protein being translated is transferred to the translocation complex (Sec61) in the ER membrane. The SRP receptor is composed of two subunits SRalpha and SRbeta. The SRalpha subunit is a translocation GTPase peripherally bound to the endoplasmic reticulum by its interaction with SRbeta. SRalpha also binds to the GTPase of SRP (SRP54) and these two proteins appear to function as each others GTPase activating proteins (GAPs). Hydrolysis of GTP by SRalpha and SRP54 is thought to be involved in transfer of the nascent protein to the Sec61 complex in the ER. SRalpha has an apparent molecular weight of 72 kDa. SRbeta is a Type I transmembrane protein that spans the membrane once and contains Ras type GTPase domain. The function of the GTPase in SRbeta is unknown. The membrane spanning domain is at the amino-terminus of SRbeta. The GTPase domain encompasses three quarters of the protein and is carboxyl- of the transmembrane region. SRalpha binds to the GTPase domain of SRbeta. Heterodimerization of SRalpha and SRbeta masks the carboxyl-terminal epitope of SRbeta.
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纯化方法
- Ammonium Sulfate Precipitation
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免疫原
- The antibody to SRalpha was raised against a recombinant protein corresponding to amino acids 39-295 which includes part of the amino terminal SRbeta binding region and the hinge region between it and the carboxyl-terminal GTPase domain.
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亚型
- IgG
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anti-Signal Recognition Particle Receptor (Docking Protein) (SRPR) (AA 1-638) antibody
SRPR 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Signal Recognition Particle Receptor (Docking Protein) (SRPR) (Center) antibodySRPR 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Signal Recognition Particle Receptor (Docking Protein) (SRPR) (AA 1-220) antibodySRPR 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Signal Recognition Particle Receptor (Docking Protein) (SRPR) (AA 1-638) antibodySRPR 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Signal Recognition Particle Receptor (Docking Protein) (SRPR) (AA 215-264) antibodySRPR 适用: 人, 小鼠, 大鼠, 豚鼠, Cow WB 宿主: 兔 Polyclonal unconjugated
anti-Signal Recognition Particle Receptor (Docking Protein) (SRPR) (AA 141-190) antibodySRPR 适用: 人, 犬, 小鼠, 大鼠, 豚鼠, 兔, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Signal Recognition Particle Receptor (Docking Protein) (SRPR) antibodySRPR 适用: 人, 犬 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- Detects SRalpha by Western blot analysis at 1 to 5 μg/mL. Also can be used to immunoprecipitate both subunits of SRP receptors under non-denaturing conditions. Optimal concentration should be evaluated by serial dilutions.
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限制
- 仅限研究用
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缓冲液
- Provided as solution in phosphate buffered saline with 0.08 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
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- SRPR (Signal Recognition Particle Receptor (Docking Protein) (SRPR))
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别名
- SR alpha
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途径
- ER-Nucleus Signaling
抗原
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