Western Blotting (WB), Immunohistochemistry (IHC), ELISA
原理
Anti-Myelin Basic Protein Mouse Monoclonal Antibody
特异性
This antibody reacts with residues 130-136 in human myelin basic protein (21.5 kDa and 18.5 kDa molecular forms). It also recognizes primate, rabbit, sheep, goat, rat, and mouse myelin basic protein.
This antibody may be used in ELISA, immunohistochemistry, and Western blot procedures for detection and/or quantitation of myelin basic protein.ELISA: Human myelin basic protein coated on ELISA wells at 5 μg/mL, binding detected with HRP-conjugated goat anti-mouse IgG + ABTS substrate.
限制
仅限研究用
状态
Liquid
溶解方式
Dilute in PBS or medium that is identical to that used in the assay system.
浓度
Lot specific
缓冲液
PBS, pH 7.4
储存条件
-20 °C,-80 °C
储存方法
This antibody is stable for at least one (1) year at -20°C to -70°C. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles.
Myelin basic protein,The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation. {PubMed:8544862}.,Myelin membrane, Peripheral membrane protein, Cytoplasmic side. Note=Cytoplasmic side of myelin., [Isoform 3]: Nucleus,MBP, Myelin A1 protein, Myelin membrane encephalitogenic protein