Fibronectin 抗体
Quick Overview for Fibronectin 抗体 (ABIN3071577)
抗原
See all Fibronectin 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Anti-Human Fibronectin Mouse Monoclonal Antibody
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特异性
- This antibody recognizes an epitope in the cell-binding domain of human and bovine fibronectin and can inhibit cell adhesion.
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交叉反应
- Cow, 人
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免疫原
- Human plasma fibronectin
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亚型
- IgG1
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anti-Fibronectin (AA 1201-1300) antibody
适用: 人, 大鼠, 小鼠, Cow WB, ELISA, ICC, IHC (p), IF (p), IHC (fro), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Fibronectin (AA 2117-2236) antibody适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Fibronectin (AA 355-558) antibody适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Fibronectin (AA 313-607) antibody适用: 人 WB, IHC, IP, FACS, ICC 宿主: 兔 Polyclonal unconjugated
anti-Fibronectin (AA 1998-2106) antibody适用: 人 WB, IHC, IP, FACS, ICC 宿主: 兔 Polyclonal unconjugated
anti-Fibronectin (AA 2240-2280) antibody适用: 人, 大鼠, 小鼠 WB, IHC (p), IF (p) 宿主: 小鼠 Monoclonal 1B9 unconjugated
anti-Fibronectin (AA 510-689) antibody适用: 小鸡 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Fibronectin (AA 355-558) antibody适用: 大鼠 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C2 unconjugated
anti-Fibronectin (AA 2206-2337) antibody适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Fibronectin antibodyKD Validated 适用: 人 WB, IHC, IF, ICC 宿主: 兔 Monoclonal unconjugated Recombinant Antibody
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应用备注
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ELISA: 1-10 μg/mL with immobilized antigen.Western Blot: 1-10 μg/mL, reducing or non-reducing conditions.Cell Adhesion Inhibition Assay: 0.1-1.0 μg/mL on 10 μg/mL fibronectin-coated microwells inhibits adhesion of trypsinized NRK cells.
Immunohistochemistry: 1-10 μg/mL, paraffin-embedded or frozen tissue sections with Proteinase K treatment for antigen retrieval.Staining of fibronectin (FN) in human well-differentiated colon cancer tissue (bottom) and moderately- differentiated colon cancer (top) with #42040.
These are recommended concentrations.
Endusers should determine optimal concentrations for their applications. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitute lyophilized antibody in 200 µL of distilled water (final concentration 2.0 mg/mL).
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缓冲液
- PBS, pH 7.4, 1 % BSA, lyophilized.
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储存条件
- 4 °C,-20 °C
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储存方法
- The stock solution (2. 0mg/ml) can be stored in aliquots at -20°C for 1 year or can be stored at 4°C for 6 months after adding 0.1% sodium azide. Prepare dilutions of stock solution immediately before use. Do not store diluted stock solution. Avoid repeated freeze-thaw cycles
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- Fibronectin
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背景
- Fibronectin,Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin (PubMed:3024962, PubMed:3900070, PubMed:3593230, PubMed:7989369). Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape (PubMed:3024962, PubMed:3900070, PubMed:3593230, PubMed:7989369). Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization (By similarity). Participates in the regulation of type I collagen deposition by osteoblasts (By similarity). Acts as a ligand for the LILRB4 receptor, inhibiting FCGR1A/CD64-mediated monocyte activation (PubMed:34089617). {UniProtKB:P11276, PubMed:3024962, PubMed:34089617, PubMed:3593230, PubMed:3900070, PubMed:7989369}., [Anastellin]: Binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling. {PubMed:11209058, PubMed:15665290, PubMed:19379667, PubMed:8114919}.,Secreted, extracellular space, extracellular matrix,FN, Cold-insoluble globulin, CIG [Cleaved into: Anastellin, Ugl-Y1, Ugl-Y2, Ugl-Y3]
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UniProt
- P02751
抗原
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