CERK 抗体
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北京 101111
Quick Overview for CERK 抗体 (ABIN306956)
抗原
See all CERK 抗体适用
宿主
克隆类型
标记
应用范围
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原理
- Rabbit anti Human Ceramide Kinase
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交叉反应
- 人
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产品特性
- Sphingolipids, in addition to being structural components of membranes, regulate cell-cell and cell-substrate interactions, proliferation, and differentiation. Members of this diverse group of lipids have emerged as a novel class of signaling molecules that also regulate phagocytosis. The mechanisms by which sphingolipids exert these effects remain incompletely defined. More than a decade ago, it was found that ceramide can be phosphorylated to ceramide 1-phosphate (C1P). Ceramide kinase (CERK) and its phosphorylated product ceramide 1-phosphate (C1P) are central players in inflammation and cancer. The product of CERK activity, ceramide 1-phosphate (C1P), has been reported to have mitogenic effects. C1P is a direct activator of cytosolic phospholipase A2 and is involved in arachidonic acid release. CERK is a mediator of Ca2+-dependent degranulation in mast cells. In both arachidonic acid release and mast cell degranulation, the intracellular elevation of Ca2+ is a central event that acts as a regulatory mechanism of CERK activity. C1P is found in brain synaptic vesicles, and plays a role in regulating the secretion of neurotransmitters. CERK activity exists in HL-60 cells where the C1P is derived from ceramide released from sphingomyelin. The expressed kinase has specific ceramide phosphorylating activity. CERKs exist in a variety of cellular organisms, including plants, nematodes, insects, and vertebrates.
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纯化方法
- Ammonium Sulfate Precipitation
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免疫原
- Synthetic peptide derived from human ceramide kinase protein
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anti-Ceramide Kinase (CERK) (AA 212-478) antibody
CERK 适用: 人 ELISA, WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Ceramide Kinase (CERK) (AA 487-516), (C-Term) antibodyCERK 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal RB5271 unconjugated
anti-Ceramide Kinase (CERK) antibodyCERK 适用: 人, 小鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ceramide Kinase (CERK) (AA 1-201) antibodyCERK 适用: 人 ELISA, WB 宿主: 小鼠 Polyclonal unconjugated
anti-Ceramide Kinase (CERK) (AA 1-201) antibodyCERK 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Ceramide Kinase (CERK) (AA 487-516) antibodyCERK 适用: 人, 小鼠 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Ceramide Kinase (CERK) (C-Term) antibodyCERK 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- Antibody can be used for Western blotting (1-10 μg/mL). Can also be used for ELISA. Optimal concentration should be evaluated by serial dilutions.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- phosphate buffered saline with 0.08 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
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- CERK (Ceramide Kinase (CERK))
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别名
- Ceramide Kinase
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背景
- Ceramide kinase, Acylsphingosine kinase, hCERK, Lipid kinase 4, LK4, CERK, KIAA1646
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UniProt
- Q8TCT0
抗原
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