FA2H 抗体
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北京 101111
Quick Overview for FA2H 抗体 (ABIN306946)
抗原
See all FA2H 抗体适用
宿主
克隆类型
标记
应用范围
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原理
- Rabbit anti Human Fatty Acid 2-hydrolase (Fa2H)
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交叉反应
- 人
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产品特性
- 2-Hydroxysphingolipids are a subset of sphingolipids containing 2-hydroxy fatty acids. The 2-hydroxylation occurs during de novo ceramide synthesis and is catalyzed by fatty acid 2-hydroxylase (also known as fatty acid alpha-hydroxylase). In mammals, 2-hydroxysphingolipids are present abundantly in brain because the major myelin lipids galactosylceramides and sulfatides contain 2-hydroxy fatty acids. Here we report identification and characterization of a human gene that encodes a fatty acid 2-hydroxylase. Data base searches revealed a human homologue of the yeast ceramide 2-hydroxylase gene (FAH1), which we named FA2H. The FA2H gene encodes a 372-amino acid protein with 36 % identity and 46 % similarity to yeast Fah1p. The amino acid sequence indicates that FA2H protein contains an N-terminal cytochrome b5 domain and four potential transmembrane domains. FA2H also contains the iron-binding histidine motif conserved among membrane-bound desaturases/hydroxylases. COS7 cells expressing human FA2H contained 3-20-fold higher levels of 2-hydroxyceramides (C16, C18, C24, and C24:1) and 2-hydroxy fatty acids compared with control cells. Microsomal fractions prepared from transfected COS7 cells showed tetracosanoic acid 2-hydroxylase activities in an NADPH- and NADPH: cytochrome P-450 reductase-dependent manner. FA2H lacking the N-terminal cytochrome b5 domain had little activity, indicating that this domain is a functional component of this enzyme. Northern blot analysis showed that the FA2H gene is highly expressed in brain and colon tissues. These results demonstrate that the human FA2H gene encodes a fatty acid 2-hydroxylase. FA2H is likely involved in the formation of myelin 2-hydroxy galactosylceramides and -sulfatides.
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纯化方法
- Ammonium Sulfate Precipitation
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免疫原
- Synthetic peptide derived from human fatty acid 2-hydrolase (FA2H) protein.
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anti-Fatty Acid 2-Hydroxylase (FA2H) (AA 121-148) antibody
FA2H 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB23881 unconjugated
anti-Fatty Acid 2-Hydroxylase (FA2H) (AA 1-372) antibodyFA2H 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Fatty Acid 2-Hydroxylase (FA2H) antibodyFA2H 适用: 人 WB 宿主: 小鼠 Monoclonal 2E3 unconjugated
anti-Fatty Acid 2-Hydroxylase (FA2H) antibodyFA2H 适用: 人 WB 宿主: 小鼠 Monoclonal 1B5 unconjugated
anti-Fatty Acid 2-Hydroxylase (FA2H) (AA 257-306) antibodyFA2H 适用: 人, 小鼠, 大鼠, Cow, 豚鼠, 兔, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Fatty Acid 2-Hydroxylase (FA2H) (C-Term) antibodyFA2H 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Fatty Acid 2-Hydroxylase (FA2H) antibodyFA2H 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
- Antibody can be used for Western blotting (5-10 μg/mL) and ELISA. Other applications no tested. Optimal concentration should be evaluated by serial dilutions.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- phosphate buffered saline with 0.08 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
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- FA2H (Fatty Acid 2-Hydroxylase (FA2H))
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别名
- Fatty Acid 2-hydrolase (Fa2H)
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背景
- Fatty acid 2-hydroxylase, FA2H
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UniProt
- Q7L5A8
抗原
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