Synaptosome-Associated Protein of 25 kDa,SNAP25 (Synaptosome-Associated Protein of 25 kDa) is a membrane bound, pre-synaptic nerve terminal protein that plays an essential role in synaptic vesicle fusion and exocytosis. It is involved in the molecular regulation of neurotransmitter release. It is thought to play an important role in the synaptic function of specific neuronal systems. SNAP25 associates with proteins involved in vesicle docking and membrane fusion. SNAP25 exists as two alternatively spliced isoforms, SNAP25A and SNAP25B. These isoforms are differentially expressed in neurons and neuroendocrine cells. These two isoforms differ by 9 amino acids in the central portion of the protein.
纯化方法
Protein A/G Chromatography
免疫原
Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with rat brain synaptosomal membranes and mouse NS1 cells.
Antibody can be used for Western blotting (1-5 μg/mL), immunocytochemistry and immunohistochemistry on paraformaldehyde fixed CNS tissues (1-5 μg/mL). Does not stain reduced proteins. Optimal concentration should be evaluated by serial dilutions.
限制
仅限研究用
缓冲液
Provided as solution in phosphate buffered saline with 0.08 % sodium azide
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C
储存方法
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles