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Myogenin 抗体 (AA 30-224)

This anti-Myogenin antibody is a 小鼠 单克隆 antibody detecting Myogenin in WB, ELISA, IF, IP, IHC (p) 和 GS. Suitable for 人.
产品编号 ABIN306849
发货至: 中国

Quick Overview for Myogenin 抗体 (AA 30-224) (ABIN306849)

抗原

See all Myogenin (MYOG) 抗体
Myogenin (MYOG) (Myogenin (Myogenic Factor 4) (MYOG))

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This Myogenin antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Gel Shift (GS)

克隆位点

F12B
  • 抗原表位

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    AA 30-224

    交叉反应

    人, 小鼠, 大鼠

    产品特性

    Myogenin, Myogenic factor 4, Myf-4,Myogenin is a member of a family of myogenic regulatory genes, which includes MyoD, myf5 and MRF4. These genes encode a set of transcription factors, which are essential for muscle development. Expression of myogenin is restricted to cells of skeletal muscle origin. It is therefore a useful marker for tumors of the muscle lineage, being strongly expressed in alveolar rhabdomyosarcomas.

    纯化方法

    Protein A/G Chromatography

    免疫原

    Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with recombinant protein containing rat myogenin amino acids 30-224 and mouse myeloma NS1 cells.

    亚型

    IgG1
  • 应用备注

    This antibody can be used for ELISA, gel supershift assays, immunofluorescence, immunoprecipitation of protein-DNA complexes (2 μg/mg), Western blotting (1 μg/mL) and is the antibody of choise for immunohistochemistry on formalin-fixed, paraffin-embedded tissue sections (1-2 μg/mL). Optimal concentration should be evaluated by serial dilutions.

    限制

    仅限研究用
  • 缓冲液

    Provided as solution in phosphate buffered saline with 0.08 % sodium azide,X1415M, clone F12B recognizes a protein of 34 kDa, identified as myogenin. Its epitope localizes between aa 158-178 of myogenin.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    -20 °C

    储存方法

    Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
  • 抗原

    Myogenin (MYOG) (Myogenin (Myogenic Factor 4) (MYOG))

    别名

    Myogenin

    途径

    Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development
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