Myoblast determination protein 1, Myogenic factor 3, MyoD1, Myf-3,X1414M monoclonal antibody, clone 5.2F recognizes a protein of 45 kDa, identified as MyoD1. The epitope of this antibody maps in between amino acid 3-56 in the N-terminus of mouse MyoD1 protein. It does not cross react with myogenin, Myf5, or Myf6. Occassionally nuclear expression/staining of MyoD1 is seen in ectomesenchymoma and a subset of Wilms tumors. Weak cytoplasmic staining/presence is observed in several non-muscle tissues, including glandular epithelium and also in rhabdomyosarcomas, neuroblastomas, Ewings sarcomas and alveolar soft part sarcomas. The 5.2F antibody to MyoD1 labels the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue.
纯化方法
Protein A/G Chromatography
免疫原
Recombinant mouse MyoD1 protein.,Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with recombinant mouse MyoD1 protein and mouse myeloma Sp2/0-Ag14 cells.
This antibody can be used for electron microscopy, ELISA, immunofluorescence, immunoprecipitation (2 μg/mg of protein lysate), Western blotting (1 μg/mL) and immunohistochemistry on frozen and formalin/paraffin fixed tissues (2-4 μg/mL). These working concentrations are merely an indication. Optimal working concentrations should, however, be evaluated by serial dilutions by the customer.
限制
仅限研究用
缓冲液
Purified monoclonal antibody 5.2F in phosphate buffered saline with 0.08 % sodium azide
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C
储存方法
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles