Kein (4,5,6,8,10,13,18) 抗体
适用: 人
IHC (fro), IHC (p), WB
宿主: 小鼠
Monoclonal
C11
unconjugated
1 image
-
- 抗原
- Kein (4,5,6,8,10,13,18)
- 适用
- 人
- 宿主
- 小鼠
- 克隆类型
- 单克隆
- 应用范围
- Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
- 交叉反应
- 人
- 产品特性
- Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinizing tissue (ie., skin) and non-keratinizing cells (ie., mesothelial cells). Although not a traditional marker for endothelial cells, cytokeratins have also been found in some microvascular endothelial cells. At least 20 different cytokeratins in the molecular range of 40-70 kDa and isoelectric points of 5-8.5 can be identified using two dimensional gel electrophoresis. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. Monoclonal antibodies to cytokeratin proteins can be useful markers for tumor identification and classification.
- 纯化方法
- Purified by saturated ammonium sulphate precipitation
- 免疫原
- Hybridoma produced by the fusion of splenocytes from mice immunized with cytoskeleton preparation from human A431 carcinoma cells and mouse myeloma cells.
- 克隆位点
- C11
- 亚型
- IgG1
-
- 应用备注
- Antibody detects keratins 4,5,6,8,10,13 & 18 by Western blot. Immunohistochemistry can be performed on frozen tissues and paraffin sections. Optimal concentration should be evaluated by serial dilutions. X1260M reacts with a variety of normal and neoplastic epithelia. Reacting with simple epithelium and both basal and superbasal layers of cornifying and non-cornifying squamous epithelium this antibody is also useful in staining cultured epithelial cell lines. It is useful in differentiating epithelial tumors from non-epithelial tumors.
- 限制
- 仅限研究用
-
- 缓冲液
- Provided as solution in phosphate buffered saline with 0.08 % sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
-
- 抗原
- Kein (4,5,6,8,10,13,18)
- UniProt
- P13647
-
You are here:
