P34 Cdc-2 Nuclear Protein 抗体
The 小鼠 单克隆 anti-P34 Cdc-2 Nuclear Protein antibody is suitable to detect P34 Cdc-2 Nuclear Protein in samples from Cow, 人, 小鼠, 大鼠 和 非洲爪蟾. It has been validated for ICC, IHC (p), IP 和 WB.
1 image
产品编号 ABIN306818
发货至:
中国
Contact our Customer Service for availability and price in your country.
Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for P34 Cdc-2 Nuclear Protein 抗体 (ABIN306818)
抗原
P34 Cdc-2 Nuclear Protein
适用
Cow, 人, 小鼠, 大鼠, 非洲爪蟾
宿主
小鼠
克隆类型
单克隆
应用范围
Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
克隆位点
POH-1
-
-
原理
- Mouse anti Human p34 cdc-2 nuclear protein
-
交叉反应
- Cow, 人, 小鼠, 大鼠, 非洲爪蟾
-
产品特性
- p34(Cdc2) kinase is a an essential nuclear protein for mitosis in human cells. During the prophase/metaphase transition the inactive phosphorylated form of p34(cdc2) is dephosphorylated to make the active form of the protein. This dephosphorylation is performed by the p80(cdc25) dual-specificity phosphatase. The regulatory subunit of p34(cdc2) is cyclin B, a 47-52 kDa protein. The p34(cdc2) protein is the catalytic subunit of the M phase-promoting factor (MPF) which is intracellular factor responsible for entry into M phase. Three mechanisms are responsible for the activitation of MPF at the onset of mitosis: binding of p34(cdc2) to cyclin B, a series of phosphorylation/dephosphorylation events on the two subunits and translocation of the complex to the nucleus or microtubules.
-
纯化方法
- Protein A/G Chromatography
-
免疫原
- Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with recombinant human p34.cdc2 fusion protein and mouse myeloma cells.
-
亚型
- IgG2a
-
-
-
应用备注
- Detects three bands within the 34 kDa region corresponding to p34 and its cleavage products. Can also be used for IHC on paraffin-embedded tissue (10 μg/mL) as well as immunocytochemistry and immunoprecipitation. Optimal concentration should be evaluated by serial dilutions.
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
缓冲液
- phosphate buffered saline with 0.08 % sodium azide
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- -20 °C
-
储存方法
- Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
-
-
- P34 Cdc-2 Nuclear Protein
-
UniProt
- P06493
抗原
-
You are here:
