RIT1 抗体
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北京 101111
Quick Overview for RIT1 抗体 (ABIN306804)
抗原
See all RIT1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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交叉反应
- 人, 小鼠
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产品特性
- Rit and its neuron-specific homologue, Rin, define a recently discovered subfamily of Ras-related GTPases. Rit and Rin are membrane-associated in spite of the fact that they lack a CAAX box or similar C-terminal lipidation motif. Rit and Rin display 64 % amino acid sequence identity and share a unique nine amino acid effector domain (DPTIEDAYK) that is 100 % conserved between the murine and human proteins. Although the effector domain sequences of Rit and Rin are very similar to that of Ras, Rit and Rin have been shown to interact with the known Ras-binding proteins RalGDS, Rlf and AF-6, but not the Raf kinases, RIN1 or the p110 subunit of PI3 kinase. For this reason, it has been suggested that Rit and Rin may play important roles in the regulation of signaling pathways distinct from those controlled by Ras.
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纯化方法
- Protein A or G purified
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免疫原
- Hybridoma produced by the fusion of splenocytes from mice immunized with recombinant full-length human Rit protein and mouse myeloma cells.
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亚型
- IgG1
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anti-Ras-Like Without CAAX 1 (RIT1) (C-Term) antibody
RIT1 适用: 人, 小鼠, 大鼠 WB, ELISA, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Ras-Like Without CAAX 1 (RIT1) (AA 1-219) antibodyRIT1 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ras-Like Without CAAX 1 (RIT1) (C-Term) antibodyRIT1 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Ras-Like Without CAAX 1 (RIT1) (AA 120-217) antibodyRIT1 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Ras-Like Without CAAX 1 (RIT1) (C-Term) antibodyRIT1 适用: 人, 小鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Ras-Like Without CAAX 1 (RIT1) (AA 108-157) antibodyRIT1 适用: 人, Cow, 兔, 绵羊, 犬, 豚鼠, 马, Bat, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Ras-Like Without CAAX 1 (RIT1) (C-Term) antibodyRIT1 适用: 人, Cow, 兔, 绵羊, 大鼠, 豚鼠, Pig, Bat WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- Antibody detects recombinant Rit protein expressed in 293 cells by Western blot analysis at a concentration of 0.5 to 1 μg/mL. Detects a band of approximately 25 kDa (predicted molecular weight: 26 kDa). Does not cross-react with Rin protein. Optimal concentration should be evaluated by serial dilutions.
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限制
- 仅限研究用
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缓冲液
- Provided as sterile filtered solution containing 20 mM sodium phosphate, 150 mM sodium chloride, 50 % glycerol at pH 7.5 and 3 mM sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
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- RIT1 (Ras-Like Without CAAX 1 (RIT1))
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别名
- Rit
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UniProt
- Q92963
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途径
- Neurotrophin Signaling Pathway
抗原
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